%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% % B-factor and local structure quality estimation % % in GPCR-I-TASSER structure modeling % %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% 1. How was the local accuracy estimated? The local accuracy was defined as the distance deviation (in Angstrom) between residue positions in the model and the native structure. It was estimated using support vector regression that makes use of the coverage of threading alignment, divergence of GPCR-I-TASSER simulation decoys, and sequence-based secondary structure and solvent accessibility predictions. Large-scale benchmark tests show that the estimated local accuracy has an average error of 2.21 Angstrom and the Pearson's correlation coefficient between estimated and actual error is 0.7. Based on these tests, the local accuracy estimations tend to be more accurate for residues: 1) that have higher threading alignment coverage 2) that are located at alpha-helix and beta-strand regions 3) that are buried (at 25% threshold) The estimated local accuracy for each model is available at the columns 61-66 in the model's PDB file and also at the bottom of this page (columns with label RSQ_*). 2. What is normalized B-factor? Normalized B-factor for a target protein is defined as z-score-based normalization of the raw B-factor values. The normalized B-factor (called B-factor profile, BFP) is predicted using a combination of both template-based assignment and profile-based prediction. Based on the distributions and predictions of the BFP, residues with BFP values higher than 2 are less stable in experimental structures. The estimated normalized B-factor is shown at the bottom of this page. For more information about the local accuracy and normalized B-factor predictions, please refer to the following article: J Yang, Y Wang, Y Zhang. ResQ: An approach to unified estimation of B-factor and residue-specific error in protein structure prediction, Journal of Molecular Biology, in press (2015). #RES SS SA COV BFP RSQ_1 RSQ_2 RSQ_3 RSQ_4 RSQ_5 1 C E 0.06 1.63 12.18 17.52 18.02 20.94 23.35 2 C E 0.08 1.14 10.96 15.70 15.96 18.58 21.80 3 C B 0.09 0.43 10.00 14.43 14.23 16.93 20.81 4 C B 0.09 0.08 9.21 13.38 12.78 14.58 20.56 5 C E 0.10 0.07 8.49 12.75 12.10 13.12 20.35 6 C E 0.13 0.09 7.83 11.75 11.41 12.09 19.58 7 C E 0.13 0.09 7.23 11.07 10.71 11.01 18.17 8 C E 0.14 -0.03 6.80 10.40 10.38 10.53 16.97 9 C E 0.14 -0.01 6.93 10.39 10.59 10.98 15.57 10 C E 0.15 0.13 6.76 10.10 10.16 9.92 14.42 11 C E 0.16 0.20 7.27 10.39 10.43 10.15 13.64 12 C E 0.16 0.13 7.19 10.31 10.25 10.25 12.36 13 C B 0.16 0.06 7.39 10.02 10.03 10.09 11.18 14 C E 0.17 0.17 6.64 9.24 9.23 9.60 9.88 15 C E 0.26 0.35 5.58 7.88 8.05 8.38 8.79 16 C E 0.29 0.39 5.17 7.66 7.77 8.11 8.15 17 C E 0.31 0.43 5.37 8.02 8.25 8.36 8.38 18 C E 0.32 0.43 5.89 8.81 8.86 8.63 9.63 19 C E 0.32 0.48 5.90 8.51 8.90 8.70 9.86 20 C E 0.41 0.44 5.63 8.09 8.34 8.37 9.18 21 C B 0.46 0.25 5.57 8.29 8.55 8.31 8.71 22 C E 0.56 0.31 4.98 7.53 8.17 7.33 8.18 23 H B 0.64 0.22 4.68 7.33 7.92 6.91 7.67 24 H B 0.71 0.08 4.71 7.13 7.99 6.92 7.50 25 H B 0.80 0.01 4.31 6.81 7.26 6.60 7.06 26 H B 0.91 -0.20 3.99 6.45 6.72 6.31 6.60 27 H B 0.95 -0.43 4.13 6.40 7.04 6.24 6.81 28 H B 0.96 -0.49 4.13 6.04 6.85 6.13 6.75 29 H B 0.98 -0.57 3.81 5.72 6.33 5.92 6.15 30 H B 0.99 -0.58 3.86 5.71 6.34 5.78 5.98 31 H B 0.99 -0.67 4.07 5.84 6.69 5.70 6.37 32 H B 0.99 -0.73 3.88 5.54 6.36 5.56 5.98 33 H B 0.99 -0.73 3.69 5.37 5.86 5.45 5.58 34 H B 0.99 -0.63 3.95 5.36 6.20 5.27 6.02 35 H E 0.99 -0.60 3.99 5.59 6.26 5.21 6.09 36 H B 0.99 -0.79 3.72 5.43 5.77 5.12 5.71 37 H B 0.99 -0.83 3.77 5.38 5.90 5.17 5.79 38 H E 0.99 -0.68 4.03 5.70 6.21 5.40 6.02 39 H E 0.99 -0.70 3.88 5.83 6.10 5.31 6.13 40 H B 0.99 -0.85 3.72 5.54 5.89 5.18 6.05 41 H B 0.99 -0.86 4.12 5.70 5.99 5.48 6.32 42 H E 0.99 -0.71 4.22 6.11 6.19 5.51 6.79 43 H B 0.99 -0.91 4.24 6.04 6.04 5.45 6.52 44 H B 0.99 -0.97 4.18 5.75 6.14 5.41 6.54 45 H E 0.99 -0.88 4.25 6.18 6.18 5.42 6.95 46 H B 0.99 -1.04 4.47 6.32 6.21 5.44 7.09 47 H B 0.99 -1.09 4.26 6.09 5.76 5.34 6.62 48 S B 0.99 -1.11 4.24 6.03 5.89 5.23 6.77 49 S B 0.99 -0.99 4.52 6.44 6.24 5.42 7.27 50 S B 0.99 -0.83 4.48 6.53 6.13 5.43 7.27 51 S B 0.99 -0.86 4.41 6.34 5.93 5.48 7.22 52 S B 0.99 -0.66 4.57 6.59 6.18 5.65 7.68 53 C B 0.99 -0.34 4.94 7.08 6.61 6.00 8.15 54 C B 0.99 -0.15 4.93 7.14 6.85 5.86 8.04 55 C E 0.99 0.10 5.43 6.92 7.01 6.43 8.46 56 C E 0.99 -0.10 5.34 6.78 6.64 6.50 8.07 57 C B 0.99 -0.34 4.98 6.43 6.25 6.09 7.75 58 C B 0.99 -0.32 4.92 6.51 6.40 6.17 7.80 59 C B 0.99 -0.56 4.70 6.15 6.07 5.97 7.78 60 H B 0.99 -0.69 4.43 5.98 5.91 5.69 7.57 61 H B 0.99 -0.84 4.17 5.77 5.76 5.57 7.21 62 H B 0.99 -0.88 4.25 5.87 5.93 5.48 7.17 63 H B 0.99 -1.00 4.28 5.81 5.94 5.58 6.94 64 H B 0.99 -0.99 4.06 5.64 5.70 5.23 6.89 65 H B 0.99 -1.00 3.94 5.56 5.64 5.25 6.49 66 H B 0.99 -1.00 4.26 5.73 5.91 5.47 6.68 67 H B 0.99 -1.08 4.21 5.69 5.71 5.52 6.60 68 H B 0.99 -1.18 3.93 5.55 5.59 5.34 6.07 69 H B 0.99 -1.07 4.10 5.62 5.86 5.37 6.30 70 H B 0.99 -1.00 4.18 5.73 5.75 5.29 6.34 71 H B 0.99 -1.05 3.83 5.42 5.47 5.26 6.12 72 H B 0.99 -0.97 3.62 5.30 5.52 5.06 5.72 73 H B 0.99 -0.98 3.82 5.69 5.71 5.20 6.07 74 H B 0.99 -0.89 3.66 5.65 5.63 5.27 5.87 75 H B 0.99 -0.91 3.53 5.25 5.34 5.16 5.45 76 H B 0.99 -0.91 3.67 5.33 5.57 5.20 5.44 77 H B 0.98 -0.92 3.70 5.65 5.75 5.32 5.87 78 H B 0.99 -0.95 3.65 5.44 5.81 5.28 6.00 79 H B 0.99 -0.94 3.36 5.37 5.48 4.96 5.62 80 H B 0.99 -0.91 3.42 5.47 5.63 5.08 5.38 81 H B 0.99 -0.82 3.57 5.73 5.80 5.32 5.66 82 H B 0.99 -0.80 3.38 5.28 5.68 5.36 5.40 83 H B 0.99 -0.81 3.20 5.20 5.60 5.04 5.27 84 H B 0.99 -0.66 3.53 5.64 5.83 5.44 5.47 85 H B 0.99 -0.58 3.58 5.76 5.98 5.81 5.58 86 H B 0.97 -0.42 3.41 5.54 5.84 5.37 5.34 87 H B 0.95 -0.36 3.52 5.83 5.99 5.41 5.31 88 H B 0.99 -0.19 3.87 6.45 6.56 5.50 5.72 89 H B 0.98 -0.07 3.92 6.37 6.83 5.69 5.85 90 C E 0.99 0.07 4.15 6.95 7.49 5.80 5.84 91 S E 0.99 -0.05 3.76 6.10 6.46 5.65 5.93 92 S B 0.99 -0.27 3.69 5.86 6.47 5.43 5.25 93 S B 0.95 -0.30 3.82 7.04 8.12 6.05 5.70 94 C B 0.96 -0.19 4.18 7.30 8.04 5.90 5.79 95 C B 0.96 -0.09 4.07 6.82 7.30 5.88 5.96 96 H E 0.94 -0.03 4.12 6.37 6.43 5.85 6.18 97 H B 0.94 -0.19 4.40 6.34 6.94 5.86 6.29 98 H B 0.99 -0.44 3.98 6.01 6.42 5.25 5.93 99 H B 0.99 -0.53 3.57 5.46 5.59 5.33 5.45 100 H B 0.99 -0.53 3.99 5.48 5.37 5.59 5.97 101 H B 0.99 -0.69 4.17 5.41 5.56 5.61 6.41 102 H B 0.99 -0.87 3.59 5.27 5.31 5.10 5.58 103 H B 0.99 -0.94 3.47 5.23 5.15 5.13 5.61 104 H B 0.99 -0.98 3.87 5.16 5.37 5.29 6.29 105 H B 0.99 -1.04 3.65 5.09 5.33 5.12 6.21 106 H B 0.99 -1.02 3.35 4.99 5.16 4.92 5.56 107 H B 0.99 -1.07 3.38 4.96 4.94 4.86 5.94 108 H B 0.99 -1.05 3.59 5.23 5.35 5.09 6.50 109 H B 0.99 -1.04 3.39 5.15 5.28 4.92 5.79 110 H B 0.99 -1.11 3.18 4.90 4.99 4.62 5.47 111 H B 0.99 -1.13 3.31 4.90 5.00 4.71 5.97 112 H B 0.99 -1.17 3.37 5.06 5.28 4.97 6.14 113 H B 0.99 -1.16 3.30 5.06 5.21 4.78 5.81 114 H B 0.99 -1.12 3.20 5.07 5.03 4.75 6.04 115 H B 0.99 -1.01 3.37 5.16 5.24 4.95 6.30 116 H B 0.99 -1.13 3.43 5.19 5.22 5.00 6.38 117 H B 0.99 -1.12 3.31 5.30 5.24 5.10 6.06 118 H B 0.99 -0.99 3.34 5.21 5.29 5.19 6.41 119 H B 0.99 -0.99 3.52 5.49 5.59 5.45 6.81 120 H B 0.99 -1.10 3.49 5.65 5.66 5.11 6.97 121 H B 0.99 -1.06 3.49 5.93 5.71 5.13 6.86 122 H B 0.99 -1.00 3.72 5.95 5.77 5.39 7.47 123 H B 0.99 -0.89 3.77 5.77 5.79 5.52 7.55 124 H B 0.99 -1.10 3.64 6.00 5.84 5.48 7.10 125 H B 0.99 -0.97 3.61 6.21 6.01 5.66 7.23 126 H B 0.99 -0.83 3.97 6.13 6.14 5.78 7.96 127 H B 0.99 -0.50 4.00 6.25 6.15 5.94 7.83 128 C B 0.99 -0.84 3.76 6.34 6.06 5.73 7.12 129 C B 0.99 -0.37 3.98 6.61 6.62 6.32 7.62 130 C B 0.99 -0.16 4.43 6.63 6.70 6.79 7.96 131 C B 0.99 -0.23 4.52 6.67 6.75 7.13 8.87 132 C B 0.98 0.06 4.80 6.82 6.93 6.25 8.16 133 C E 0.98 0.29 5.25 6.62 6.91 6.80 8.81 134 C B 0.99 0.19 4.40 6.96 6.81 6.71 8.27 135 C E 0.99 0.27 4.92 6.87 6.86 6.38 8.65 136 C E 0.99 0.20 5.28 7.08 6.88 7.66 9.39 137 C E 0.99 -0.01 4.74 6.44 6.44 5.98 8.46 138 C B 0.99 -0.08 4.41 5.92 5.86 5.88 7.70 139 C B 0.99 -0.01 5.01 6.19 6.30 6.43 8.08 140 H E 0.99 -0.24 4.95 6.17 6.39 6.23 8.01 141 H B 0.99 -0.26 5.15 6.11 6.67 6.43 8.07 142 H B 0.99 -0.47 4.85 5.86 6.50 6.08 7.99 143 H B 0.99 -0.55 4.50 5.72 6.13 5.71 7.91 144 H B 0.99 -0.52 4.68 5.69 6.45 5.77 7.82 145 H B 0.99 -0.56 4.69 5.72 6.58 5.87 7.97 146 H B 0.99 -0.71 4.40 5.62 6.14 5.76 7.69 147 H B 0.99 -0.74 4.22 5.48 5.98 5.45 7.24 148 H B 0.99 -0.76 4.39 5.52 6.30 5.60 7.35 149 H B 0.99 -0.81 4.32 5.63 6.13 5.69 7.47 150 H B 0.99 -0.92 3.90 5.52 5.85 5.40 7.18 151 H B 0.99 -0.87 3.92 5.41 5.93 5.38 7.11 152 H B 0.99 -0.71 4.26 5.74 5.94 5.65 7.46 153 H B 0.99 -0.80 3.97 5.60 5.54 5.40 7.32 154 H B 0.99 -0.85 3.73 5.29 5.29 5.03 6.87 155 H B 0.99 -0.74 3.89 5.41 5.53 5.15 7.03 156 H E 0.99 -0.69 4.15 5.57 5.59 5.40 7.16 157 H B 0.99 -0.70 3.95 5.50 5.30 5.17 6.91 158 H B 0.99 -0.63 3.71 5.25 5.29 5.10 6.54 159 H B 0.99 -0.57 3.92 5.44 5.32 5.45 6.89 160 H B 0.99 -0.62 4.20 6.31 5.76 5.83 7.03 161 H B 0.99 -0.56 3.98 5.97 5.56 5.50 6.92 162 H B 0.99 -0.48 3.90 5.72 5.38 5.48 7.02 163 H B 0.96 -0.32 4.29 6.36 5.89 6.08 6.94 164 C B 0.98 -0.14 4.37 6.54 5.91 6.28 6.99 165 C B 0.98 -0.08 4.16 6.41 5.98 6.20 7.31 166 C E 0.96 0.14 4.08 7.92 6.53 5.61 7.11 167 C E 0.92 0.40 4.18 9.35 7.48 6.03 7.35 168 C E 0.90 0.59 4.87 10.22 8.30 6.91 8.06 169 C E 0.87 0.70 4.87 9.57 8.67 7.12 8.00 170 C E 0.84 0.77 5.05 9.11 8.69 7.17 8.26 171 C E 0.84 0.74 4.93 7.76 7.75 6.77 7.82 172 C E 0.91 0.63 4.72 7.03 7.11 6.40 7.10 173 C E 0.93 0.30 4.80 6.75 7.13 6.37 6.51 174 C E 0.89 -0.06 4.48 6.18 6.65 6.13 6.18 175 S B 0.86 -0.28 3.74 5.47 5.94 5.47 5.41 176 S B 0.91 -0.40 3.43 5.25 5.01 4.83 5.18 177 S B 0.91 -0.28 3.37 5.27 5.03 4.76 5.34 178 S B 0.91 -0.33 3.66 5.45 5.31 5.12 5.67 179 S B 0.90 -0.25 3.78 6.06 6.25 5.13 6.11 180 S E 0.93 -0.17 3.91 5.84 5.45 5.28 6.62 181 C E 0.99 -0.32 4.22 6.39 6.76 5.51 7.20 182 C E 0.99 -0.18 4.20 6.11 5.75 5.47 7.05 183 H B 0.99 -0.50 4.55 6.87 6.99 5.62 7.11 184 H B 0.99 -0.74 4.22 6.74 7.01 5.38 6.97 185 H B 0.99 -0.83 3.82 6.14 5.77 5.23 6.60 186 H B 0.99 -0.76 3.88 5.73 5.40 5.31 6.74 187 H B 0.99 -0.86 3.90 5.74 5.53 5.44 6.77 188 H B 0.99 -0.90 3.67 5.69 5.39 5.33 6.67 189 H B 0.99 -0.94 3.59 5.33 5.33 5.20 6.37 190 H B 0.99 -0.92 3.72 5.27 5.36 5.27 6.57 191 H B 0.99 -0.87 3.76 5.57 5.57 5.40 6.82 192 H B 0.99 -0.93 3.54 5.70 5.30 5.30 6.49 193 H B 0.99 -0.98 3.24 5.23 4.99 4.97 6.57 194 H E 0.99 -0.78 3.39 5.17 5.41 5.30 6.49 195 H B 0.99 -0.85 3.70 5.20 5.52 5.65 6.83 196 H B 0.99 -0.94 3.51 5.20 5.19 5.17 6.75 197 H B 0.99 -0.91 3.37 5.24 5.20 5.25 6.71 198 H B 0.99 -0.94 3.65 5.35 5.70 5.92 6.97 199 H B 0.99 -1.03 3.57 5.36 5.47 5.64 7.15 200 H B 0.99 -1.00 3.42 5.31 5.15 5.45 7.00 201 H B 0.99 -0.86 3.43 5.45 5.42 5.73 6.90 202 H E 0.99 -0.71 3.62 5.46 5.67 5.90 7.35 203 H B 0.99 -0.90 3.60 5.67 5.60 5.89 7.48 204 H B 0.99 -0.92 3.44 5.68 5.49 5.86 6.93 205 H B 0.99 -0.82 3.71 5.77 5.85 6.30 6.89 206 H B 0.99 -0.77 3.71 5.77 5.75 6.24 7.16 207 H B 0.99 -0.87 3.72 5.88 5.70 6.04 7.06 208 H B 0.99 -0.77 3.81 6.12 5.87 6.16 6.58 209 H B 0.99 -0.57 3.94 6.22 6.03 6.64 6.95 210 H B 0.99 -0.55 4.06 6.19 5.99 6.68 7.40 211 H B 0.99 -0.58 4.05 6.26 6.14 6.42 7.07 212 H B 0.99 -0.40 4.21 6.39 6.16 6.86 6.88 213 H E 0.99 -0.29 4.25 6.63 6.18 7.09 7.32 214 H E 0.99 -0.25 4.18 6.54 6.35 6.64 7.58 215 H E 0.94 -0.15 4.23 6.19 5.95 7.05 7.64 216 H E 0.90 -0.11 4.37 8.24 6.75 7.09 7.55 217 H E 0.81 -0.09 4.55 8.18 7.02 7.20 8.19 218 H E 0.79 -0.06 4.68 8.72 7.32 6.83 8.09 219 C E 0.76 -0.02 5.00 9.03 7.35 7.09 7.90 220 C E 0.74 -0.07 5.22 8.90 7.47 7.51 8.30 221 C E 0.74 -0.05 5.42 9.30 7.89 7.75 8.71 222 C E 0.73 -0.01 5.78 9.75 8.37 7.37 8.72 223 C E 0.73 -0.01 6.75 10.36 8.90 9.37 10.51 224 C E 0.72 0.08 7.50 10.39 9.40 9.55 11.44 225 C E 0.70 0.12 8.05 11.16 11.05 9.61 12.03 226 C E 0.62 0.16 8.75 12.87 12.55 11.24 13.35 227 C E 0.61 0.25 9.08 14.82 14.13 12.54 15.19 228 C E 0.59 0.26 10.06 15.67 15.48 12.81 17.31 229 C E 0.57 0.28 10.17 15.11 15.24 13.23 16.77 230 C E 0.57 0.27 10.18 15.83 16.58 15.94 17.87 231 C E 0.57 0.24 10.57 16.62 17.53 16.54 19.15 232 C E 0.57 0.14 10.43 17.11 19.18 15.74 18.25 233 C E 0.57 0.10 9.99 16.31 18.16 17.09 18.11 234 C E 0.57 0.12 9.74 15.78 17.17 17.23 17.58 235 C E 0.57 0.15 9.89 15.42 16.78 15.91 16.19 236 C E 0.57 0.13 9.60 14.99 16.60 15.26 15.97 237 C E 0.57 0.13 8.88 13.14 14.19 16.17 15.46 238 C E 0.57 0.27 8.66 12.86 12.93 14.50 13.90 239 C E 0.57 0.37 8.94 12.97 13.45 13.96 13.83 240 C E 0.57 0.38 7.69 10.95 11.92 13.47 13.01 241 C E 0.57 0.33 6.25 9.84 9.35 13.41 11.85 242 C E 0.57 0.32 5.81 10.57 8.80 13.18 11.73 243 C E 0.57 0.26 6.47 10.42 8.76 12.99 12.36 244 C E 0.57 0.22 6.40 9.76 8.89 11.83 11.68 245 C E 0.57 0.12 6.14 8.72 8.26 9.23 9.83 246 C B 0.57 0.03 6.16 9.46 8.51 7.76 8.97 247 C E 0.57 -0.02 6.16 10.92 9.41 7.99 9.84 248 C E 0.64 -0.07 5.98 10.79 8.91 8.52 10.03 249 C E 0.64 -0.07 5.92 9.88 9.19 10.63 10.12 250 C B 0.64 -0.18 5.96 11.24 9.87 11.04 10.78 251 C E 0.64 -0.13 5.92 12.87 9.92 11.29 11.29 252 C E 0.64 -0.13 5.84 12.39 9.77 11.82 10.78 253 C B 0.64 -0.16 5.69 11.16 9.95 11.67 10.25 254 C E 0.64 -0.14 5.69 12.57 10.36 10.15 10.42 255 C E 0.69 -0.09 5.59 13.78 10.01 9.52 10.27 256 C E 0.73 -0.08 5.37 12.83 9.80 10.21 9.85 257 C E 0.75 -0.13 5.24 11.89 9.99 8.93 9.04 258 C E 0.76 -0.14 5.19 12.89 10.70 7.35 8.85 259 C E 0.79 -0.15 5.05 14.41 11.29 6.83 9.32 260 H E 0.79 -0.18 4.89 12.92 10.09 6.54 8.53 261 H E 0.82 -0.19 4.81 11.05 9.07 6.59 8.32 262 H B 0.84 -0.08 4.80 12.08 9.65 6.60 8.42 263 H E 0.85 -0.15 4.72 11.11 8.98 6.12 8.17 264 H B 0.89 -0.05 4.39 8.27 7.12 5.89 7.40 265 H E 0.94 0.07 4.41 9.03 7.83 6.19 7.72 266 H E 0.99 0.03 4.44 9.08 7.69 6.33 7.72 267 H E 0.99 -0.13 4.10 8.01 6.78 5.86 7.16 268 H E 0.99 -0.18 4.01 6.04 5.73 5.99 7.12 269 H E 0.99 -0.45 3.98 6.09 5.86 6.02 7.33 270 H B 0.99 -0.63 3.97 5.96 5.77 5.69 6.91 271 H B 0.99 -0.73 3.81 5.64 5.56 5.48 6.65 272 H E 0.99 -0.61 3.78 5.62 5.33 5.57 6.73 273 H B 0.99 -0.71 3.79 5.64 5.69 5.36 6.54 274 H B 0.99 -0.78 3.62 5.57 5.61 5.21 6.36 275 H B 0.99 -0.80 3.53 5.51 5.28 5.60 6.33 276 H B 0.99 -0.89 3.54 5.50 5.42 5.30 6.00 277 H B 0.99 -0.93 3.50 5.43 5.47 5.42 5.80 278 H B 0.99 -0.82 3.18 5.33 5.16 5.16 5.67 279 H B 0.99 -0.75 3.18 5.39 5.23 5.27 5.99 280 H B 0.99 -0.81 3.13 5.25 5.25 4.91 5.76 281 H B 0.99 -0.86 3.10 5.28 5.09 5.02 5.47 282 H B 0.99 -0.73 3.07 5.41 5.19 4.90 5.77 283 H E 0.99 -0.82 3.21 5.36 5.35 5.03 5.80 284 H B 0.99 -0.91 3.14 5.03 5.06 4.80 5.50 285 H B 0.99 -1.00 3.06 5.23 4.83 4.88 5.49 286 H B 0.99 -0.92 3.30 5.10 4.93 4.83 5.73 287 H B 0.99 -0.99 3.43 5.24 5.05 4.93 5.64 288 H B 0.99 -1.14 3.24 5.24 4.85 4.72 5.60 289 H B 0.99 -0.99 3.30 5.20 4.88 4.92 5.68 290 H B 0.99 -0.82 3.71 5.41 5.04 5.11 5.88 291 H B 0.99 -0.78 3.69 5.57 5.16 4.97 5.96 292 H B 0.99 -0.80 3.49 5.48 5.12 5.20 6.05 293 H B 0.99 -0.65 3.89 5.57 5.28 5.35 6.56 294 H B 0.99 -0.52 4.10 5.82 5.55 5.33 6.65 295 H B 0.97 -0.30 4.04 5.94 5.62 5.14 6.60 296 H B 0.96 -0.26 4.20 5.96 5.71 5.37 6.75 297 H B 0.96 -0.13 4.52 6.68 6.11 5.46 7.30 298 C B 0.90 0.03 4.69 7.15 6.60 5.90 7.52 299 C B 0.84 0.28 4.91 7.43 6.73 6.11 7.97 300 C E 0.82 0.50 5.08 8.03 7.39 6.50 8.46 301 C B 0.81 0.34 4.86 7.44 6.58 5.89 7.82 302 C B 0.61 0.14 5.01 7.11 6.89 6.10 7.95 303 C B 0.88 -0.06 4.23 6.27 6.19 5.35 7.00 304 H B 0.94 -0.07 4.20 6.26 6.60 5.20 6.60 305 H E 0.99 -0.11 3.82 5.90 6.33 4.96 5.69 306 H B 0.96 -0.29 3.96 5.90 6.45 4.95 5.82 307 H B 0.98 -0.51 3.76 5.61 5.82 4.81 5.74 308 H B 0.99 -0.69 3.31 5.22 5.43 4.67 5.55 309 H B 0.99 -0.68 3.44 5.31 5.59 4.97 5.47 310 H B 0.99 -0.76 3.53 5.45 5.58 4.74 5.43 311 H B 0.99 -0.86 3.24 5.38 5.23 4.71 5.44 312 H B 0.99 -0.87 3.09 5.07 5.11 4.90 5.25 313 H B 0.99 -0.91 3.31 5.10 5.22 4.98 5.41 314 H B 0.99 -0.88 3.21 5.24 5.16 4.62 5.28 315 H B 0.99 -0.94 3.11 5.06 5.05 4.65 5.34 316 H B 0.99 -0.87 3.18 5.11 5.35 4.67 5.19 317 H E 0.99 -0.85 3.23 5.33 5.29 4.65 5.11 318 H B 0.99 -1.00 3.11 5.32 5.27 4.91 5.15 319 H B 0.99 -0.99 3.55 5.29 5.38 5.25 5.47 320 H B 0.99 -0.94 3.65 5.44 5.72 5.03 5.62 321 H E 0.99 -0.75 3.66 5.70 5.48 5.28 5.57 322 H B 0.99 -0.90 3.58 5.47 5.42 5.25 5.51 323 H B 0.99 -0.99 3.94 5.89 5.83 5.35 5.84 324 H B 0.99 -0.90 4.05 6.03 6.18 5.85 6.13 325 H B 0.99 -0.89 4.00 5.88 5.84 5.92 6.08 326 H B 0.99 -0.73 4.15 5.81 5.73 5.76 6.08 327 H B 0.99 -0.57 4.49 6.37 6.43 6.05 6.62 328 H B 0.86 -0.48 4.73 6.86 6.76 6.77 6.89 329 C B 0.84 -0.48 4.55 6.81 6.78 6.80 6.88 330 C B 0.99 -0.35 4.46 6.09 6.06 6.25 6.78 331 H E 0.99 -0.15 4.74 6.39 6.36 6.24 7.21 332 H B 0.99 -0.19 4.92 6.41 6.30 6.03 7.71 333 H B 0.99 -0.41 4.55 6.24 6.11 5.66 7.21 334 H B 0.99 -0.33 4.65 6.64 6.31 5.89 7.20 335 H E 0.99 -0.10 4.95 7.10 6.70 5.96 8.01 336 H B 0.99 -0.31 4.98 6.82 6.60 5.85 8.08 337 H B 0.99 -0.43 4.81 6.72 6.49 5.63 7.55 338 H B 0.99 -0.25 5.13 6.98 6.89 5.95 8.16 339 H E 0.97 -0.21 5.33 7.43 7.21 6.16 8.45 340 H B 0.96 -0.34 5.29 7.19 7.08 6.18 8.44 341 H B 0.93 -0.25 5.37 7.22 7.23 6.08 8.46 342 C B 0.84 -0.03 5.79 7.83 7.85 6.80 9.15 343 C B 0.64 0.10 6.20 8.30 8.48 7.44 9.67 344 C B 0.38 0.22 6.19 8.42 8.67 7.67 10.03 345 C B 0.21 0.12 6.27 8.62 8.82 7.80 9.98 346 C B 0.19 0.53 6.69 9.16 9.54 8.43 10.80 347 C B 0.14 0.80 6.75 8.97 9.51 8.81 10.79 348 C E 0.12 0.90 7.04 9.31 9.79 8.96 11.27 349 C E 0.09 0.66 7.19 9.84 10.37 9.54 11.77 350 C E 0.09 0.65 7.03 9.79 10.45 9.58 11.47 351 C E 0.08 0.50 7.13 9.85 10.13 9.49 11.12 352 C E 0.07 0.53 7.50 10.24 10.46 9.84 11.80 353 C E 0.06 0.55 7.54 10.70 11.02 10.26 12.00 354 C E 0.06 0.41 7.37 10.81 10.87 10.20 11.51 355 C E 0.03 0.28 7.76 11.52 11.43 10.44 11.88 356 C E 0.03 0.26 8.21 11.68 11.45 10.65 12.46 357 C E 0.03 0.33 8.66 12.26 12.18 11.93 13.61 358 C E 0.01 0.30 8.85 12.72 13.25 12.62 13.93 359 C E 0.01 0.46 9.12 13.94 14.48 13.23 14.09 360 C B 0.01 0.63 9.44 14.70 15.46 14.01 14.48 361 C E 0.01 0.45 9.51 15.27 16.56 14.78 14.05 362 C E 0.01 0.48 10.19 16.42 18.03 16.43 13.88 363 C E 0.01 0.43 10.56 16.90 18.79 17.68 13.25 364 C E 0.01 0.49 10.52 16.78 20.53 19.05 12.61 365 C E 0.01 0.60 10.40 16.89 20.60 19.70 13.19 366 C E 0.01 0.23 10.82 17.31 19.95 18.94 13.18 367 C E 0.00 -0.03 11.07 17.81 20.53 19.27 12.77 368 C E 0.00 0.01 10.95 18.01 21.73 20.89 14.26 369 C E 0.00 0.01 10.78 18.32 22.37 21.13 15.93 370 C E 0.01 0.38 10.79 18.55 23.43 21.18 16.77 371 C E 0.01 0.41 10.82 18.81 23.71 22.30 17.41 372 C E 0.01 0.43 11.45 19.15 24.20 22.10 18.25 373 C E 0.01 0.50 11.42 19.17 23.29 20.20 18.34 374 C E 0.01 0.64 10.78 18.67 22.93 19.53 16.84 375 C E 0.01 0.70 10.45 18.10 22.19 19.99 15.97 376 C E 0.02 0.53 11.23 18.46 22.55 21.30 15.88 377 C E 0.02 0.47 11.47 19.60 23.26 22.40 16.62 378 C E 0.02 0.53 12.84 21.20 24.15 24.71 17.52 379 C E 0.02 0.60 13.58 23.08 25.25 25.67 18.76 380 C E 0.01 1.05 14.80 25.41 26.59 27.50 20.12 381 C E 0.01 0.96 15.89 26.67 27.05 28.39 20.96 382 C E 0.01 0.37 16.64 28.08 27.35 28.43 21.96 383 C E 0.01 0.39 16.15 27.29 25.83 26.33 22.18 384 C B 0.01 0.22 14.93 25.42 24.07 24.54 21.32 385 C E 0.01 0.18 13.92 23.55 22.10 23.55 20.21 386 C E 0.01 0.27 13.59 21.67 21.19 22.97 20.13 387 C E 0.01 0.32 13.84 21.09 20.62 22.78 20.21 388 C E 0.01 0.27 13.71 19.90 19.63 22.81 20.15 389 C E 0.01 0.33 14.53 20.55 20.58 23.66 21.62 390 C E 0.00 0.22 15.11 19.83 21.05 24.36 23.23 391 C E 0.01 0.44 15.89 20.36 21.77 24.64 24.60 392 C E 0.01 0.54 16.52 20.24 21.40 24.64 25.62 393 C E 0.01 0.48 16.34 20.13 20.46 23.61 25.33 394 C E 0.00 0.10 15.63 19.67 19.05 21.66 23.84 395 C E 0.00 0.01 15.08 19.19 18.76 19.57 22.51 396 C E 0.00 0.04 14.85 20.18 19.90 18.88 23.09 397 C E 0.00 0.00 14.60 20.04 20.27 17.37 22.91 398 C E 0.00 0.01 14.91 21.82 21.66 16.78 24.15 399 C B 0.00 -0.01 15.23 22.28 21.44 15.59 23.73 400 C E 0.00 0.03 15.59 22.94 22.97 15.72 24.42 401 C E 0.01 0.43 16.11 24.39 23.77 16.64 25.74 402 C E 0.01 0.47 16.82 25.30 24.18 17.59 26.35 403 C E 0.01 0.40 17.04 25.66 23.75 18.43 26.55 404 C E 0.01 0.39 17.13 26.61 23.95 19.18 27.48 405 C E 0.00 0.18 17.21 26.59 24.57 20.09 27.87 406 C E 0.00 0.28 16.81 26.30 23.86 20.42 28.13 407 C E 0.01 0.53 16.98 26.29 23.78 21.26 28.47 408 C E 0.01 0.45 16.61 25.67 22.96 21.39 27.87 409 C E 0.01 0.59 15.92 24.56 22.79 21.19 26.34 410 C E 0.01 0.55 14.82 23.57 21.46 20.33 24.36 411 C E 0.01 0.50 13.92 23.40 22.17 19.58 23.31 412 C E 0.01 0.50 13.24 23.40 22.53 18.23 21.69 413 C E 0.01 0.57 13.34 23.76 24.29 17.73 21.31 414 C E 0.01 0.51 13.69 24.70 25.65 16.19 20.90 415 C E 0.01 0.49 13.62 24.22 26.10 13.87 20.00 416 C E 0.01 0.48 13.89 24.10 26.97 12.43 19.37 417 C E 0.01 0.39 13.69 23.77 26.87 10.82 18.71 418 C E 0.01 0.33 14.12 24.04 26.96 10.93 18.59 419 C E 0.01 0.35 14.40 23.11 25.98 12.18 18.07 420 C E 0.01 0.27 14.94 23.15 25.44 14.41 18.01 421 C E 0.01 0.21 15.38 22.71 24.45 16.90 18.16 422 H E 0.00 -0.04 15.31 22.29 23.79 18.74 17.93 423 H E 0.00 -0.06 15.58 21.68 24.38 21.00 18.15 424 C B 0.00 -0.12 15.42 20.34 23.93 20.79 17.66 425 C E 0.00 0.04 16.56 20.65 25.61 22.79 18.51 426 C E 0.00 0.06 17.28 21.45 25.88 23.95 19.59 427 C E 0.00 0.03 17.82 21.70 25.39 25.32 20.00 428 C E 0.00 -0.03 17.65 21.72 24.11 25.71 20.02 429 C E 0.00 -0.16 16.51 22.13 22.25 24.19 18.76 430 C E 0.00 -0.13 15.73 21.93 20.45 22.86 18.59 431 C E 0.00 -0.18 15.76 21.74 19.92 22.12 18.32 432 C E 0.00 -0.15 15.08 18.88 18.87 21.08 18.27 433 C B 0.00 -0.25 14.36 16.06 18.24 19.82 18.32 434 C B 0.00 -0.28 13.90 13.84 17.89 19.38 18.68 435 C B 0.00 -0.30 13.24 12.42 18.20 19.08 19.29 436 C B 0.00 -0.10 12.75 12.80 19.31 18.57 20.17 437 C B 0.00 0.01 13.04 14.23 20.84 18.61 20.61 438 C B 0.00 0.04 13.45 16.37 22.26 19.09 21.32 439 C E 0.00 0.11 14.11 17.87 23.64 19.96 21.67 440 C E 0.00 0.22 14.35 19.82 24.27 21.16 21.75 441 C E 0.00 0.25 14.82 21.93 25.18 22.83 21.81 442 C E 0.00 0.27 15.35 23.46 25.31 23.96 21.63 443 C E 0.00 0.32 15.74 25.22 25.19 23.90 21.10 444 C E 0.00 0.44 17.23 26.87 26.14 25.47 21.68 445 C E 0.00 0.39 18.14 27.83 26.32 27.00 22.00 446 C E 0.00 0.28 17.99 27.51 24.81 26.95 21.46 447 C E 0.00 0.13 17.15 27.12 23.29 25.80 21.34 448 C E 0.00 0.15 16.93 27.40 21.84 25.25 21.59 449 C B 0.00 -0.03 16.00 25.72 19.80 23.69 21.89 450 C B 0.00 -0.19 15.87 24.03 18.38 22.33 22.09 451 S E 0.00 -0.16 14.89 22.99 16.99 20.37 22.18 452 S B 0.00 -0.23 14.15 20.98 16.81 19.12 21.59 453 S E 0.00 -0.13 13.89 21.64 16.18 18.32 22.48 454 S E 0.00 -0.21 13.57 20.93 17.78 17.64 22.04 455 S B 0.00 -0.22 14.73 20.74 19.80 17.16 22.99 456 S E 0.00 -0.08 15.47 21.68 22.17 17.61 23.80 457 S B 0.00 -0.18 15.95 21.41 24.36 18.91 23.83 458 C E 0.00 -0.02 16.82 23.28 26.48 19.35 25.17 459 C B 0.00 -0.09 16.79 23.91 27.56 20.70 25.13 460 C E 0.00 0.28 17.86 26.26 29.74 21.47 27.41 461 C E 0.00 0.47 18.59 27.80 31.48 22.44 28.98 462 C E 0.00 0.56 19.30 29.14 32.16 23.10 29.93 463 C E 0.00 0.60 19.49 29.38 31.88 23.25 29.90 464 C E 0.00 0.85 19.88 29.44 31.83 23.82 29.91 465 C E 0.01 1.35 20.15 29.30 31.50 24.57 29.76 466 C E 0.02 2.24 20.19 28.84 31.00 25.40 29.19 RES: Residue number SS: Predicted secondary structure: C - random coil; H - alpha-helix; S - beta-strand SA: Predicted solevent accessibility at 25% cutoff: E - exposed; B - buried COV: Threading alignment coverage defined as the number of threading alignments on the residue divided by the number of total threading programs BFP: Predicted normalized B-factor RSQ_*: Residue-Specific Quality of models defined as the estimated deviation of the residue on the model from the native structure of the protein You are requested to cite the following article when you use the local structure quality prediction: J Yang, Y Wang, Y Zhang. ResQ: An approach to unified estimation of B-factor and residue-specific error in protein structure prediction, Journal of Molecular Biology, in press (2015).