D-I-TASSER O14793

D-I-TASSER results for O14793

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: O14793
Protein Name: Growth/differentiation factor 8
Gene Name: MSTN

Input Sequence in FASTA format

>O14793 (375 residues)
MQKLQLCVYIYLFMLIVAGPVDLNENSEQKENVEKEGLCNACTWRQNTKSSRIEAIKIQI
LSKLRLETAPNISKDVIRQLLPKAPPLRELIDQYDVQRDDSSDGSLEDDDYHATTETIIT
MPTESDFLMQVDGKPKCCFFKFSSKIQYNKVVKAQLWIYLRPVETPTTVFVQILRLIKPM
KDGTRYTGIRSLKLDMNPGTGIWQSIDVKTVLQNWLKQPESNLGIEIKALDENGHDLAVT
FPGPGEDGLNPFLEVKVTDTPKRSRRDFGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIA
PKRYKANYCSGECEFVFLQKYPHTHLVHQANPRGSAGPCCTPTKMSPINMLYFNGKEQII
YGKIPAMVVDRCGCS

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of DeepPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	O14793-D1	1-119,259-375	236		MQKLQLCVYIYLFMLIVAGPVDLNENSEQKENVEKEGLCNACTWRQNTKSSRIEAIKIQILSKLRLETAPNISKDVIRQLLPKAPPLRELIDQYDVQRDDSSDGSLEDDDYHATTETIIDTPKRSRRDFGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGECEFVFLQKYPHTHLVHQANPRGSAGPCCTPTKMSPINMLYFNGKEQIIYGKIPAMVVDRCGCS
2	O14793-D2	120-258	139		TMPTESDFLMQVDGKPKCCFFKFSSKIQYNKVVKAQLWIYLRPVETPTTVFVQILRLIKPMKDGTRYTGIRSLKLDMNPGTGIWQSIDVKTVLQNWLKQPESNLGIEIKALDENGHDLAVTFPGPGEDGLNPFLEVKVT

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.72

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of DeepPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	5ntuA	0.43	1.96	0.531	0.451	model1_5ntuA.pdb.gz
2	6h3iA	0.43	5.97	0.051	0.632	model1_6h3iA.pdb.gz
3	5szsA	0.41	6.52	0.056	0.659	model1_5szsA.pdb.gz
4	3rjrA	0.40	5.71	0.128	0.571	model1_3rjrA.pdb.gz
5	6w98A2	0.40	6.19	0.044	0.616	model1_6w98A2.pdb.gz
6	5e5wA	0.39	5.85	0.053	0.589	model1_5e5wA.pdb.gz
7	1flcA	0.39	5.81	0.046	0.587	model1_1flcA.pdb.gz
8	7kc4B	0.39	5.56	0.068	0.557	model1_7kc4B.pdb.gz
9	4a7kA	0.38	6.44	0.064	0.611	model1_4a7kA.pdb.gz
10	7auwA	0.37	5.89	0.049	0.552	model1_7auwA.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0005102	0.98	receptor binding
GO:0005160	0.94	transforming growth factor beta receptor binding
GO:0005125	0.74	cytokine activity

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0065007	0.99	biological regulation
GO:0050794	0.98	regulation of cellular process
GO:0044699	0.88	single-organism process
GO:0009987	0.74	cellular process
GO:0048518	0.70	positive regulation of biological process
GO:0032502	0.70	developmental process
GO:0031323	0.70	regulation of cellular metabolic process
GO:0080090	0.69	regulation of primary metabolic process
GO:0060255	0.69	regulation of macromolecule metabolic process
GO:0048522	0.69	positive regulation of cellular process
GO:0044763	0.69	single-organism cellular process
GO:0048519	0.68	negative regulation of biological process
GO:0044767	0.68	single-organism developmental process
GO:0048523	0.67	negative regulation of cellular process
GO:0031325	0.67	positive regulation of cellular metabolic process
GO:0007165	0.67	signal transduction
GO:0051239	0.66	regulation of multicellular organismal process
GO:0010604	0.66	positive regulation of macromolecule metabolic process
GO:0048583	0.65	regulation of response to stimulus
GO:0050793	0.64	regulation of developmental process
GO:2000026	0.63	regulation of multicellular organismal development
GO:0045595	0.62	regulation of cell differentiation
GO:0051246	0.61	regulation of protein metabolic process
GO:0051241	0.61	negative regulation of multicellular organismal process
GO:0023051	0.61	regulation of signaling
GO:0010646	0.61	regulation of cell communication
GO:0048869	0.60	cellular developmental process
GO:0048584	0.60	positive regulation of response to stimulus
GO:0032268	0.60	regulation of cellular protein metabolic process
GO:0023056	0.60	positive regulation of signaling
GO:0019220	0.60	regulation of phosphate metabolic process
GO:0010647	0.60	positive regulation of cell communication
GO:0009966	0.60	regulation of signal transduction
GO:0048856	0.59	anatomical structure development
GO:0009967	0.59	positive regulation of signal transduction
GO:0001934	0.59	positive regulation of protein phosphorylation
GO:0042127	0.58	regulation of cell proliferation
GO:1902531	0.57	regulation of intracellular signal transduction
GO:0043408	0.56	regulation of MAPK cascade
GO:0008285	0.54	negative regulation of cell proliferation
GO:0042981	0.53	regulation of apoptotic process
GO:0010862	0.53	positive regulation of pathway-restricted SMAD protein phosphorylation

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044421	0.97	extracellular region part
GO:0005615	0.91	extracellular space
GO:0044464	0.71	cell part
GO:0044424	0.57	intracellular part

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	1gpeA	0.336	6.84	0.032	0.565	1.1.3.4	NA
2	0.060	3hz3A	0.340	6.74	0.047	0.571	2.4.1.5	NA
3	0.060	1fa9A	0.321	7.14	0.027	0.555	2.4.1.1	NA
4	0.060	2vtfA	0.373	6.81	0.042	0.619	3.2.1.96	NA
5	0.060	3cl4A	0.374	6.19	0.040	0.584	3.1.1.53	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

No Binding sites was predicted

Click to view	Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Qiqige Wuyun, Yang Li, Quancheng Liu, Xiaogen Zhou, Yiheng Zhu, P. Lydia Freddolino, Yang Zhang. Integrating deep learning potentials with I-TASSER for single- and multi-domain protein structure prediction. Submitted. 2023.