D-I-TASSER O60477-D1

D-I-TASSER results for O60477-D1

[Click result.zip to download all results on this page]

Input Sequence in FASTA format

>O60477 (376 residues)
MNWRFVELLYFLFIWGRISVQPSHQEPAGTDQHVSKEFDWLISDRGPFHHSRSYLSFVER
HRQGFTTRYKIYREFARWKVRNTAIERRDLVRHPVPLMPEFQRSIRLLGRRPTTQQFIDT
IIKKYGTHLLISATLGGEEALTMYMDKSRLDRKSGNATQSVEALHQLASSYFVDRDGTMR
RLHEIQISTGAIKVTETRTGPLGCNSYDNLDSVSSVLLQSTESKLHLQGLQIIFPQYLQE
KFVQSALSYIMCNGEGEYLCQNSQCRCQCAEEFPQCNCPITDIQIMEYTLANMAKSWAEA
YKDLENSDEFKSFMKRLPSNHFLTIGSIHQHWGNDWDLQNRYKLLQSATEAQRQKIQRTA
RKLFGLSVRCRHNPNH

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MNWRFVELLYFLFIWGRISVQPSHQEPAGTDQHVSKEFDWLISDRGPFHHSRSYLSFVERHRQGFTTRYKIYREFARWKVRNTAIERRDLVRHPVPLMPEFQRSIRLLGRRPTTQQFIDTIIKKYGTHLLISATLGGEEALTMYMDKSRLDRKSGNATQSVEALHQLASSYFVDRDGTMRRLHEIQISTGAIKVTETRTGPLGCNSYDNLDSVSSVLLQSTESKLHLQGLQIIFPQYLQEKFVQSALSYIMCNGEGEYLCQNSQCRCQCAEEFPQCNCPITDIQIMEYTLANMAKSWAEAYKDLENSDEFKSFMKRLPSNHFLTIGSIHQHWGNDWDLQNRYKLLQSATEAQRQKIQRTARKLFGLSVRCRHNPNH
Prediction	CCHHHHHHHHHHHHHHHHHCCSCCCCCCCCCCCCCCCCCSSSCCCCCCCCCHHHHHHHHHHHHCCSSSSSSSSCCCCCSHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHSCCCCCCSSSSSSSCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCSSSSSSCCCCCCCCCCCCCCCCHHHSSSCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHSSCCCSSSSSSCCCSSSSCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
Confidence	9424999999999988631101112456667788877443551788755687999999998625416788874122100214566665403688888927878778751466167777899999744421101347815899983606652013775144999999987732673368999999987524058773367887888767644201101128403455555899949999999999998872237871699828955786599998889998999999999999999999998988632999999985871111567899999833667999999999999999999999999998875540469899
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MNWRFVELLYFLFIWGRISVQPSHQEPAGTDQHVSKEFDWLISDRGPFHHSRSYLSFVERHRQGFTTRYKIYREFARWKVRNTAIERRDLVRHPVPLMPEFQRSIRLLGRRPTTQQFIDTIIKKYGTHLLISATLGGEEALTMYMDKSRLDRKSGNATQSVEALHQLASSYFVDRDGTMRRLHEIQISTGAIKVTETRTGPLGCNSYDNLDSVSSVLLQSTESKLHLQGLQIIFPQYLQEKFVQSALSYIMCNGEGEYLCQNSQCRCQCAEEFPQCNCPITDIQIMEYTLANMAKSWAEAYKDLENSDEFKSFMKRLPSNHFLTIGSIHQHWGNDWDLQNRYKLLQSATEAQRQKIQRTARKLFGLSVRCRHNPNH
Prediction	6312001000000000100021234444346642433011002341214404401501431453131103014313403045221433432523130244025201101332325411530044103300121324232000000336405544543431141035112321143631053035132332204034344231303434314212111241362403031012000410363004100110003340210046440104046612602013420430351044025104422541452640340044023421031210231032434034105403410440253034003301300430464388
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Contact, Hydrogen and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

Hydrogen bond networks

D-I-TASSER simulation is guided by the consensus contact map (left figure), distance map (middle figure) and Hydrogen bond network (right figure) derived based on confidence scores of DeepPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Top 10 threading templates used by D-I-TASSER

Rank

PDB
hit

ID1

ID2

Cov

Norm.
Zscore

Download
alignment

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

SS
Seq

CCHHHHHHHHHHHHHHHHHCCSCCCCCCCCCCCCCCCCCSSSCCCCCCCCCHHHHHHHHHHHHCCSSSSSSSSCCCCCSHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHSCCCCCCSSSSSSSCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCSSSSSSCCCCCCCCCCCCCCCCHHHSSSCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHSSCCCSSSSSSCCCSSSSCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
MNWRFVELLYFLFIWGRISVQPSHQEPAGTDQHVSKEFDWLISDRGPFHHSRSYLSFVERHRQGFTTRYKIYREFARWKVRNTAIERRDLVRHPVPLMPEFQRSIRLLGRRPTTQQFIDTIIKKYGTHLLISATLGGEEALTMYMDKSRLDRKSGNATQSVEALHQLASSYFVDRDGTMRRLHEIQISTGAIKVTETRTGPLGCNSYDNLDSVSSVLLQSTESKLHLQGLQIIFPQYLQEKFVQSALSYIMCNGEGEYLCQNSQCRCQCAEEFPQCNCPITDIQIMEYTLANMAKSWAEAYKDLENSDEFKSFMKRLPSNHFLTIGSIHQHWGNDWDLQNRYKLLQSATEAQRQKIQRTARKLFGLSVRCRHNPNH

3t5oA

0.12

0.11

3.98

1.14

SPARKS-K

GGICKTVKSSRTSNPYRVPANLENVGFEVQTAEDDLKTDFYSSKRSENINHNSFKQAIQASHK---KDSSFIRIHKVMKVLNFTTK-----AKDLHLSDVFLKALNHLPLEYNS-ALYSRIFDDFGTHYFTSGSLGGVYDLLYQFSSEELKNSGLTEEEAKHCVRIETKKRVLFAKTTNKLSEKHEGSFIQGAISLIRGGRWLESVKENPAVIDFELANIPCAVTKRNNLRKALQEYAAKF---DPCQCAPCPNNGRPTLSGTECLCVCQSGTYGENCEKQSPDYKSNA---VDGQWGCWSSWSTCDATYKRSRTRECNNPAPQRGGKRCEGEKRQEEDCTPCINDDEEDSGCPQPVPPENGFIRNEKQLYLVGED

3ojyB

0.16

0.10

3.27

1.50

CNFpred

----------------------------------------------------RGKHYIRRTKRFSHTKSVFLHARSDLEVAHYKLKPR-----SLMLHYEFLQRVKRLPLEYS-YGEYRDLFRDFGTHYITEAVLGGIYEYTLVMNKEAMERGDYTLNNVHACAK-----SVGKCRGILNEIKDRNKMMVEDLVVLVRGGAWGDAVQYNPAIIKVKVEP-AYSSTVRQNMKQALEEFQKEVS---SCHCAPCQGNGVPVLKGSRCDCICPVGSQGLACEVSYR-----KNTPIDGKWNCWSN--------------------------------------------------------------------------

2pffB

0.07

0.06

2.70

1.05

MapAlign

FFVSVRKAITVLFFIGVRCYVNKTNSHLPAGKQVEISLVNGAKNLVVSGPPQSLYGLNLTLRGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGKAAQDVWNRADNHFKDTYGFSILDIVINNPLTIHFGGEKGKRIRENYSEKGLLSATQFTQPALTLMEKAAFEDLKLVEVVFYRGMTMQV-------

3t5oA

0.14

0.11

3.90

4.16

HHsearch

VPANL------------ENVGFEVQTAKDLTSLGHVPI--FYSSKRSEN-INHNSAFKQAIQASHKKDSSFIRIHKVMKVLNFTTK-----AKDLHLSDVFLKALNHLPLEYNSA-LYSRIFDDFGTHYFTSGSLGGVYDLLYQFSSEELKNSGLTE----EEAKHCVKRVLFAKEHRCTTLSEKHEGSAALAWEEKTFSEWLESVKENPAVIDFELVNIPCAVTKRNNLRKALQEYAAKFD---PCQCAPCPNNGRPTLSGTECLCVCQSGTYGENCEKQSPD---YKSNAVDGQWGCWSSWSTCDATYKRSRTRAPQTPCINDDE--------------------------------------EDSGCPQPVPP

3i0pA

0.09

3.33

0.59

CEthreader

------QTKNVSIDTIKEFMYQVLLKVGSDEENARMVRDTLIAADLRGMDTHGIQRFKTVYIDRIKKGMINPTAKPSIIRETSTTCVLDGNNGFHVNGTIGMKMAIEKAKKYGMGMVVVRNSTHFGIAGYYSLLAAQEGCIGICGTNARSSVAATFGDEPILGTNPLAIGIPSDEAFPYCFDGATSISPTGRFEKYVRMG--------KTVDKSWASMKGGKPIEDPKELLENYPKGKAYLHPLGGSDEVSGSHKGYCLSEFVEIMSSCLSIA-NFLNHIEEEKEKSGKFSLGHFFIAINVECFRDLNEFKKNVGDI-NRTLRNTDKLPGHDRIYTAGEKEYETEQKRRKFGDDLPLVTINEMKELSSFYNVPLPF

6bk8U

0.07

0.06

2.57

0.85

EigenThreader

ESTDEAELINVLLVKGFTSHILERYQQKRNESLATLALNITIKSVYEKYLLNFNYLASLEKLGDNQYEEFMRQMNGPD--KWLFLILSLAKYYIGRLDSCGDLLKKSLQQFDRIYNFYLLFEQECSQFILGKLKEND---------DNDVALRQDSLVETWMKRVSLQKSAA--EKCNVYSEAILKIDPRKVGTPGSFGRLAISTARELWTQSLKVPYPYIEDLEEIYLNWADRELDVERAFSILEDALHVPTNPEI------------LLESLRIWSKYIDYLEAYIFNKTKMAYNTVIDLRLQLWRMCISKAESTLGPSVTRELYQECIQILPNSKAVEFVIKFSDFESSIGREILAYGAKLL-----PPSRNT

6cxoA

0.15

0.10

3.49

0.88

FFAS-3D

----HLEVFKAINREKTSNFNADFALKFSATEVPEFKF-----------SYFMGKNFRRLSSYFSQSKKMFVHLRGVVQLGRFVMRNRD-----VVLRSTFLDDVKALPTSYEKGEYFG-FLETYGTHYSTSGSLGGQYEIVYVLDKASMKEKGVDLNDVKHCLDVISFIRGGTREQAILLKEKILRGDKTFDKTDFANWASSLANAPALISQRMSPIKIKDAYIKKQNLEKAVEDYIDEFSTKRCY---PCLNGGTIILLDGQCLCSCPMMFRGMACEIHQ----------------------------------------------------------------------------------------------

6cxoA

0.15

0.10

3.49

1.12

SPARKS-K

TENYDEHLEVFKAINREKTSNFNADF--------------ALKFSATEVPSYFMGKNFRRLSSYFSQKKMFVHLRGVVQLGRFVMR-----NRDVVLRSTFLDDVKALPTSYEK-GEYFGFLETYGTHYSTSGSLGGQYEIVYVLDKASMKEKGVDLNDVKHCLDNIIDDVISFTREQAILLKEKILRGDK-TFDKTDFANWASSLANAPALISQRMSPIKDAYIKKQNLEKAVEDYIDEFS---TKRCYPCLNGGTIILLDGQCLCSCPMMFRGMACEIHQ----------------------------------------------------------------------------------------------

3ojyA

0.18

0.11

3.47

1.36

CNFpred

-------------------------------------------------------SFLNELNKYNEKKFIFTRIFTKVQTAHFKMRKD-----DIMLDEGMLQSLMELPDQYN-YGMYAKFINDYGTHYITSGSMGGIYEYILVIDKAKMESLGITSRDITTCFGGSLG-CKKFGGGKTERARKAMAVEDIISRVR-TYRSWGRSLKYNPVVIDFEMQP-GPLEAKRQNLRRALDQYLMEFN---ACRCGPCFNNGVPILEGTSCRCQCRLGSLGAACE-----------AKADGSWSCWSS--------------------------------------------------------------------------

5cskA

0.05

0.04

1.88

0.83

DEthreader

SERLQTKSGGYDNQVIMNASLQ------------KNNLILAILKHY-QPLC--SSKVSAQGALPSVERTIYVVFDLLYIRAYRAYIWKFQLPLREGILMALANVCVAST--EE-ILVRLRILDLNKLIIRNGNIPALELRGI-------------------EYLTSEANRLMDI-FIAVFDIPDVEAAFGGFKTEKPGSMHL--RPIATP-YP--------RYAHLGTTYVDITFKIGSFGQRIGMAEE-KENSVLTE------VIKTI------IGSEDGLGVECLRGSVGIGAYLVRGQRAIQYGSHLT-AV--D-----D---LAGVEKIVEWMSYSFFRARGVIGVMILANWRIELRAGVLEQGMVGIFR--

(a)	ID1 is the number of template residues identical to query divided by number of aligned residues.
(b)	ID2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment and is highlighted in bold.
(e)	Download alignment lists the threading program used to identify the template, and provide the 3D structure of aligned regions of threading templates (threading[1-10].pdb.gz).
(f)	Template residues identical to query sequence are highlighted in color.

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.4304

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of DeepPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	3t5oA	0.64	4.81	0.114	0.840	model1_3t5oA.pdb.gz
2	3ojyB	0.50	5.56	0.113	0.721	model1_3ojyB.pdb.gz
3	6cxoA	0.50	4.89	0.145	0.670	model1_6cxoA.pdb.gz
4	6u23A	0.48	5.61	0.091	0.694	model1_6u23A.pdb.gz
5	3nsjA1	0.48	5.03	0.097	0.652	model1_3nsjA1.pdb.gz
6	3ojyA	0.48	5.74	0.106	0.710	model1_3ojyA.pdb.gz
7	2qp2A	0.43	5.97	0.073	0.649	model1_2qp2A.pdb.gz
8	2qqhA	0.40	4.68	0.114	0.535	model1_2qqhA.pdb.gz
9	3ifsA	0.24	6.82	0.045	0.402	model1_3ifsA.pdb.gz
10	1uw4B	0.23	5.75	0.022	0.340	model1_1uw4B.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0003824	0.30	catalytic activity

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0071300	1.00	cellular response to retinoic acid
GO:0045930	1.00	negative regulation of mitotic cell cycle
GO:0045666	1.00	positive regulation of neuron differentiation

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0043025	1.00	neuronal cell body
GO:0030425	1.00	dendrite
GO:0005783	1.00	endoplasmic reticulum

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	1uyvA	0.305	6.66	0.033	0.497	6.3.4.14 6.4.1.2	NA
2	0.060	1lf6A	0.352	6.29	0.050	0.556	3.2.1.3	138
3	0.060	3ebgA	0.357	6.56	0.047	0.567	3.4.11.-	NA
4	0.060	1fo4A	0.341	6.84	0.039	0.572	1.17.1.4	NA
5	0.060	2sqcA	0.351	6.55	0.039	0.577	5.4.99.17	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.01	3ojyB	0.502	5.55	0.116	0.721	0.26	BMA	complex1.pdb.gz	43,77,78
2	0.01	1t01A	0.219	5.79	0.049	0.322	0.17	III	complex2.pdb.gz	45,52,55,56,62,199
3	0.01	1syqA	0.226	5.88	0.074	0.335	0.17	III	complex3.pdb.gz	52,56,59,60,62,63,66
4	0.01	1xwjA	0.218	5.88	0.044	0.325	0.16	III	complex4.pdb.gz	45,52,55,56,58,59,62
5	0.01	1zvzA	0.186	5.62	0.037	0.271	0.16	III	complex5.pdb.gz	37,38,42,45,85
6	0.01	1zw3A	0.190	5.25	0.038	0.269	0.13	III	complex6.pdb.gz	46,47,59,60,63
7	0.01	2x2iC	0.314	7.03	0.040	0.540	0.25	QPS	complex7.pdb.gz	64,65,252,256,257
8	0.01	2xejA	0.354	6.34	0.032	0.553	0.12	OKD	complex8.pdb.gz	71,256,257,270
9	0.01	1zw2A	0.224	5.97	0.035	0.335	0.15	III	complex9.pdb.gz	47,53,54,57,60,61,64
10	0.01	2xvkA	0.382	6.79	0.040	0.630	0.15	FFX	complex10.pdb.gz	59,64,65,253,259

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Qiqige Wuyun, Yang Li, Quancheng Liu, Xiaogen Zhou, Yiheng Zhu, P. Lydia Freddolino, Yang Zhang. Integrating deep learning potentials with I-TASSER for single- and multi-domain protein structure prediction. Submitted. 2023.