D-I-TASSER O95166

D-I-TASSER results for O95166

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: O95166
Protein Name: Gamma-aminobutyric acid receptor-associated protein
Gene Name: GABARAP

Input Sequence in FASTA format

>O95166 (117 residues)
MKFVYKEEHPFEKRRSEGEKIRKKYPDRVPVIVEKAPKARIGDLDKKKYLVPSDLTVGQF
YFLIRKRIHLRAEDALFFFVNNVIPPTSATMGQLYQEHHEEDFFLYIAYSDESVYGL

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of DeepPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	O95166-D1	1-117	117		MKFVYKEEHPFEKRRSEGEKIRKKYPDRVPVIVEKAPKARIGDLDKKKYLVPSDLTVGQFYFLIRKRIHLRAEDALFFFVNNVIPPTSATMGQLYQEHHEEDFFLYIAYSDESVYGL

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.9408

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of DeepPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	1eo6B	0.95	1.33	0.573	1.000	model1_1eo6B.pdb.gz
2	2zjdC	0.92	1.69	0.308	0.992	model1_2zjdC.pdb.gz
3	1kotA	0.90	1.77	1.000	1.000	model1_1kotA.pdb.gz
4	5azhA	0.89	1.50	0.310	0.966	model1_5azhA.pdb.gz
5	2zpnA	0.89	1.28	0.550	0.940	model1_2zpnA.pdb.gz
6	4eoyA	0.87	1.55	0.398	0.966	model1_4eoyA.pdb.gz
7	3h9dA	0.87	1.84	0.474	0.974	model1_3h9dA.pdb.gz
8	3vvwB	0.85	1.43	0.355	0.940	model1_3vvwB.pdb.gz
9	3w1yA	0.78	2.03	0.327	0.906	model1_3w1yA.pdb.gz
10	4gdkA	0.70	1.19	0.230	0.744	model1_4gdkA.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0008092	0.63	cytoskeletal protein binding
GO:0015631	0.60	tubulin binding
GO:0008017	0.56	microtubule binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0044699	0.99	single-organism process
GO:0009987	0.99	cellular process
GO:0044763	0.98	single-organism cellular process
GO:0050896	0.95	response to stimulus
GO:0033554	0.94	cellular response to stress
GO:0009605	0.94	response to external stimulus
GO:0007154	0.94	cell communication
GO:0006995	0.93	cellular response to nitrogen starvation
GO:0006914	0.62	autophagy
GO:0016043	0.61	cellular component organization
GO:0006996	0.59	organelle organization
GO:0070925	0.58	organelle assembly
GO:0007033	0.58	vacuole organization
GO:1902589	0.57	single-organism organelle organization
GO:0000045	0.57	autophagosome assembly
GO:0000422	0.56	mitophagy

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044444	1.00	cytoplasmic part
GO:0044446	0.97	intracellular organelle part
GO:0016020	0.96	membrane
GO:0098805	0.94	whole membrane
GO:0044437	0.94	vacuolar part
GO:0005774	0.93	vacuolar membrane
GO:0005829	0.88	cytosol
GO:0000421	0.85	autophagosome membrane

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	2wdqB	0.449	4.15	0.082	0.709	1.3.99.1	NA
2	0.060	1vraB	0.515	3.83	0.076	0.821	2.3.1.1 2.3.1.35	NA
3	0.060	2nptD	0.505	2.79	0.099	0.650	2.7.11.25	NA
4	0.060	1t3tA	0.448	4.79	0.044	0.821	6.3.5.3	NA
5	0.060	1u0uA	0.442	4.63	0.088	0.803	2.3.1.-	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.01	2d11C	0.560	2.73	0.123	0.692	0.16	III	complex1.pdb.gz	20,53,54,55,59
2	0.01	2d11B	0.557	2.78	0.123	0.692	0.13	III	complex2.pdb.gz	77,78,79,80,81
3	0.01	2d10A	0.558	2.75	0.123	0.692	0.11	III	complex3.pdb.gz	53,54,55,59
4	0.01	3bz3A	0.355	4.76	0.000	0.667	0.18	YAM	complex4.pdb.gz	42,43,44,45,60,61,63,77

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Qiqige Wuyun, Yang Li, Quancheng Liu, Xiaogen Zhou, Yiheng Zhu, P. Lydia Freddolino, Yang Zhang. Integrating deep learning potentials with I-TASSER for single- and multi-domain protein structure prediction. Submitted. 2023.