D-I-TASSER P10912

D-I-TASSER results for P10912

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: P10912
Protein Name: Growth hormone receptor
Gene Name: GHR

Input Sequence in FASTA format

>P10912 (638 residues)
MDLWQLLLTLALAGSSDAFSGSEATAAILSRAPWSLQSVNPGLKTNSSKEPKFTKCRSPE
RETFSCHWTDEVHHGTKNLGPIQLFYTRRNTQEWTQEWKECPDYVSAGENSCYFNSSFTS
IWIPYCIKLTSNGGTVDEKCFSVDEIVQPDPPIALNWTLLNVSLTGIHADIQVRWEAPRN
ADIQKGWMVLEYELQYKEVNETKWKMMDPILTTSVPVYSLKVDKEYEVRVRSKQRNSGNY
GEFSEVLYVTLPQMSQFTCEEDFYFPWLLIIIFGIFGLTVMLFVFLFSKQQRIKMLILPP
VPVPKIKGIDPDLLKEGKLEEVNTILAIHDSYKPEFHSDDSWVEFIELDIDEPDEKTEES
DTDRLLSSDHEKSHSNLGVKDGDSGRTSCCEPDILETDFNANDIHEGTSEVAQPQRLKGE
ADLLCLDQKNQNNSPYHDACPATQQPSVIQAEKNKPQPLPTEGAESTHQAAHIQLSNPSS
LSNIDFYAQVSDITPAGSVVLSPGQKNKAGMSQCDMHPEMVSLCQENFLMDNAYFCEADA
KKCIPVAPHIKVESHIQPSLNQEDIYITTESLTTAAGRPGTGEHVPGSEMPVPDYTSIHI
VQSPQGLILNATALPLPDKEFLSSCGYVSTDQLNKIMP

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of DeepPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	P10912-D1	1-149	149		MDLWQLLLTLALAGSSDAFSGSEATAAILSRAPWSLQSVNPGLKTNSSKEPKFTKCRSPERETFSCHWTDEVHHGTKNLGPIQLFYTRRNTQEWTQEWKECPDYVSAGENSCYFNSSFTSIWIPYCIKLTSNGGTVDEKCFSVDEIVQP
2	P10912-D2	150-294	145		DPPIALNWTLLNVSLTGIHADIQVRWEAPRNADIQKGWMVLEYELQYKEVNETKWKMMDPILTTSVPVYSLKVDKEYEVRVRSKQRNSGNYGEFSEVLYVTLPQMSQFTCEEDFYFPWLLIIIFGIFGLTVMLFVFLFSKQQRIK
3	P10912-D3	295-638	344		MLILPPVPVPKIKGIDPDLLKEGKLEEVNTILAIHDSYKPEFHSDDSWVEFIELDIDEPDEKTEESDTDRLLSSDHEKSHSNLGVKDGDSGRTSCCEPDILETDFNANDIHEGTSEVAQPQRLKGEADLLCLDQKNQNNSPYHDACPATQQPSVIQAEKNKPQPLPTEGAESTHQAAHIQLSNPSSLSNIDFYAQVSDITPAGSVVLSPGQKNKAGMSQCDMHPEMVSLCQENFLMDNAYFCEADAKKCIPVAPHIKVESHIQPSLNQEDIYITTESLTTAAGRPGTGEHVPGSEMPVPDYTSIHIVQSPQGLILNATALPLPDKEFLSSCGYVSTDQLNKIMP

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.501

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of DeepPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	3hhrB	0.30	1.31	0.990	0.306	model1_3hhrB.pdb.gz
2	1vw1A	0.29	7.96	0.036	0.472	model1_1vw1A.pdb.gz
3	6h3iA	0.29	8.49	0.020	0.489	model1_6h3iA.pdb.gz
4	3l5hA	0.28	4.83	0.102	0.345	model1_3l5hA.pdb.gz
5	5fvmA	0.28	8.06	0.034	0.461	model1_5fvmA.pdb.gz
6	8avbB	0.28	4.85	0.066	0.346	model1_8avbB.pdb.gz
7	7zolB	0.28	8.36	0.034	0.480	model1_7zolB.pdb.gz
8	3ew3B	0.28	2.65	0.293	0.302	model1_3ew3B.pdb.gz
9	3qmzA	0.28	8.14	0.023	0.464	model1_3qmzA.pdb.gz
10	4i18R	0.28	2.83	0.294	0.304	model1_4i18R.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0017046	0.65	peptide hormone binding
GO:0004871	0.53	signal transducer activity
GO:0004872	0.52	receptor activity
GO:0004888	0.51	transmembrane signaling receptor activity

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0065007	0.86	biological regulation
GO:0050789	0.85	regulation of biological process
GO:0050794	0.83	regulation of cellular process
GO:0048518	0.77	positive regulation of biological process
GO:0019222	0.76	regulation of metabolic process
GO:0080090	0.75	regulation of primary metabolic process
GO:0060255	0.75	regulation of macromolecule metabolic process
GO:0048522	0.75	positive regulation of cellular process
GO:0031323	0.75	regulation of cellular metabolic process
GO:0007165	0.75	signal transduction
GO:0031325	0.74	positive regulation of cellular metabolic process
GO:0010604	0.74	positive regulation of macromolecule metabolic process
GO:0031399	0.73	regulation of protein modification process
GO:0019220	0.73	regulation of phosphate metabolic process
GO:0001934	0.72	positive regulation of protein phosphorylation
GO:0007166	0.69	cell surface receptor signaling pathway
GO:0050896	0.66	response to stimulus
GO:0010033	0.64	response to organic substance
GO:0009987	0.63	cellular process
GO:0060396	0.61	growth hormone receptor signaling pathway
GO:0044699	0.59	single-organism process
GO:0008152	0.54	metabolic process
GO:0009725	0.53	response to hormone
GO:1901700	0.52	response to oxygen-containing compound
GO:0050731	0.52	positive regulation of peptidyl-tyrosine phosphorylation
GO:0071704	0.50	organic substance metabolic process
GO:0044237	0.50	cellular metabolic process

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044464	0.95	cell part
GO:0016020	0.92	membrane
GO:0044424	0.80	intracellular part
GO:0044444	0.70	cytoplasmic part
GO:0005829	0.54	cytosol

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	1eulA	0.219	7.71	0.041	0.345	3.6.3.8	NA
2	0.060	3b9jJ	0.126	7.19	0.024	0.193	1.17.1.4 1.17.3.2	NA
3	0.060	1ofdA	0.242	7.85	0.014	0.386	1.4.7.1	NA
4	0.060	2vkzG	0.241	8.23	0.042	0.397	2.3.1.38 3.1.2.14	NA
5	0.060	1t3tA	0.233	7.57	0.035	0.364	6.3.5.3	108

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.01	1ea0B	0.229	7.93	0.030	0.365	0.13	FMN	complex1.pdb.gz	41,42,139
2	0.01	1ea0A	0.255	7.69	0.026	0.398	0.12	FMN	complex2.pdb.gz	59,60,238,239,240,241
3	0.01	1bqu0	0.264	3.74	0.192	0.302	0.18	III	complex3.pdb.gz	75,77,130,131,133
4	0.01	3fmg2	0.130	6.33	0.050	0.185	0.19	III	complex4.pdb.gz	71,73,74,75
5	0.01	2vdcB	0.231	8.30	0.017	0.386	0.16	OMT	complex5.pdb.gz	147,148,242
6	0.01	2vdcB	0.231	8.30	0.017	0.386	0.14	FMN	complex6.pdb.gz	41,238,239

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Qiqige Wuyun, Yang Li, Quancheng Liu, Xiaogen Zhou, Yiheng Zhu, P. Lydia Freddolino, Yang Zhang. Integrating deep learning potentials with I-TASSER for single- and multi-domain protein structure prediction. Submitted. 2023.