D-I-TASSER P14866

D-I-TASSER results for P14866

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: P14866
Protein Name: Heterogeneous nuclear ribonucleoprotein L
Gene Name: HNRNPL

Input Sequence in FASTA format

>P14866 (589 residues)
MSRRLLPRAEKRRRRLEQRQQPDEQRRRSGAMVKMAAAGGGGGGGRYYGGGSEGGRAPKR
LKTDNAGDQHGGGGGGGGGAGAAGGGGGGENYDDPHKTPASPVVHIRGLIDGVVEADLVE
ALQEFGPISYVVVMPKKRQALVEFEDVLGACNAVNYAADNQIYIAGHPAFVNYSTSQKIS
RPGDSDDSRSVNSVLLFTILNPIYSITTDVLYTICNPCGPVQRIVIFRKNGVQAMVEFDS
VQSAQRAKASLNGADIYSGCCTLKIEYAKPTRLNVFKNDQDTWDYTNPNLSGQGDPGSNP
NKRQRQPPLLGDHPAEYGGPHGGYHSHYHDEGYGPPPPHYEGRRMGPPVGGHRRGPSRYG
PQYGHPPPPPPPPEYGPHADSPVLMVYGLDQSKMNCDRVFNVFCLYGNVEKVKFMKSKPG
AAMVEMADGYAVDRAITHLNNNFMFGQKLNVCVSKQPAIMPGQSYGLEDGSCSYKDFSES
RNNRFSTPEQAAKNRIQHPSNVLHFFNAPLEVTEENFFEICDELGVKRPSSVKVFSGKSE
RSSSGLLEWESKSDALETLGFLNHYQMKNPNGPYPYTLKLCFSTAQHAS

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of DeepPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	P14866-D1	1-589	589		MSRRLLPRAEKRRRRLEQRQQPDEQRRRSGAMVKMAAAGGGGGGGRYYGGGSEGGRAPKRLKTDNAGDQHGGGGGGGGGAGAAGGGGGGENYDDPHKTPASPVVHIRGLIDGVVEADLVEALQEFGPISYVVVMPKKRQALVEFEDVLGACNAVNYAADNQIYIAGHPAFVNYSTSQKISRPGDSDDSRSVNSVLLFTILNPIYSITTDVLYTICNPCGPVQRIVIFRKNGVQAMVEFDSVQSAQRAKASLNGADIYSGCCTLKIEYAKPTRLNVFKNDQDTWDYTNPNLSGQGDPGSNPNKRQRQPPLLGDHPAEYGGPHGGYHSHYHDEGYGPPPPHYEGRRMGPPVGGHRRGPSRYGPQYGHPPPPPPPPEYGPHADSPVLMVYGLDQSKMNCDRVFNVFCLYGNVEKVKFMKSKPGAAMVEMADGYAVDRAITHLNNNFMFGQKLNVCVSKQPAIMPGQSYGLEDGSCSYKDFSESRNNRFSTPEQAAKNRIQHPSNVLHFFNAPLEVTEENFFEICDELGVKRPSSVKVFSGKSERSSSGLLEWESKSDALETLGFLNHYQMKNPNGPYPYTLKLCFSTAQHAS

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.5036

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of DeepPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	4n0tA	0.56	1.37	0.150	0.577	model1_4n0tA.pdb.gz
2	3ecqB	0.39	7.38	0.048	0.601	model1_3ecqB.pdb.gz
3	6v8pA	0.37	7.48	0.039	0.587	model1_6v8pA.pdb.gz
4	5ijoJ	0.37	7.68	0.034	0.584	model1_5ijoJ.pdb.gz
5	6ez8A	0.36	8.14	0.058	0.604	model1_6ez8A.pdb.gz
6	2zxqA	0.36	7.59	0.037	0.571	model1_2zxqA.pdb.gz
7	4kx7A	0.35	8.00	0.057	0.593	model1_4kx7A.pdb.gz
8	6rc9A	0.35	7.73	0.039	0.572	model1_6rc9A.pdb.gz
9	5mu1A	0.35	7.84	0.042	0.579	model1_5mu1A.pdb.gz
10	6eqoA	0.35	7.36	0.056	0.547	model1_6eqoA.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0003723	1.00	RNA binding
GO:0044822	0.94	poly(A) RNA binding
GO:0003729	0.71	mRNA binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0009987	0.90	cellular process
GO:0065007	0.71	biological regulation
GO:0050789	0.70	regulation of biological process
GO:0008152	0.68	metabolic process
GO:0050794	0.67	regulation of cellular process
GO:0071704	0.66	organic substance metabolic process
GO:0044699	0.66	single-organism process
GO:0043170	0.65	macromolecule metabolic process
GO:0060255	0.64	regulation of macromolecule metabolic process
GO:0044237	0.63	cellular metabolic process
GO:0080090	0.62	regulation of primary metabolic process
GO:0044260	0.62	cellular macromolecule metabolic process
GO:0044238	0.62	primary metabolic process
GO:0031323	0.62	regulation of cellular metabolic process
GO:0010468	0.62	regulation of gene expression
GO:0051171	0.61	regulation of nitrogen compound metabolic process
GO:0044763	0.61	single-organism cellular process
GO:0034641	0.60	cellular nitrogen compound metabolic process
GO:0016070	0.59	RNA metabolic process
GO:0006396	0.52	RNA processing

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044424	0.99	intracellular part
GO:0044446	0.83	intracellular organelle part
GO:0044428	0.81	nuclear part
GO:0032991	0.68	macromolecular complex
GO:0005654	0.66	nucleoplasm
GO:0043226	0.63	organelle
GO:0043229	0.62	intracellular organelle
GO:0043227	0.61	membrane-bounded organelle
GO:0030529	0.61	intracellular ribonucleoprotein complex
GO:0043231	0.60	intracellular membrane-bounded organelle
GO:0005634	0.58	nucleus

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	2hpiA	0.291	7.99	0.050	0.482	2.7.7.7	174
2	0.060	2a4zA	0.320	7.95	0.035	0.530	2.7.1.153	NA
3	0.060	2rd0A	0.327	8.12	0.047	0.542	2.7.1.153	138
4	0.060	1sllA	0.312	7.91	0.038	0.516	4.2.2.15	NA
5	0.060	1b0pA	0.356	7.29	0.055	0.560	1.2.7.1	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.03	3q43A	0.330	7.73	0.047	0.536	0.16	D66	complex1.pdb.gz	141,142,146,149,150,153,154,157
2	0.02	2wxiA	0.347	7.84	0.027	0.569	0.21	S30	complex2.pdb.gz	106,130,140,141,142
3	0.02	2wxnA	0.345	7.86	0.046	0.569	0.17	DLN	complex3.pdb.gz	107,162,163,164,165
4	0.01	2wxjA	0.344	7.78	0.034	0.560	0.16	RW3	complex4.pdb.gz	106,108,113
5	0.01	2zxgA	0.336	7.91	0.042	0.557	0.12	S23	complex5.pdb.gz	154,157,158,164,165
6	0.01	3iayA	0.343	7.61	0.045	0.552	0.15	DCP	complex6.pdb.gz	114,115,116
7	0.01	3b34A	0.338	7.78	0.047	0.552	0.14	PHE	complex7.pdb.gz	138,140,141
8	0.01	2wxlA	0.333	8.20	0.040	0.565	0.19	ZS4	complex8.pdb.gz	104,106,142
9	0.01	3suoA	0.283	8.04	0.037	0.467	0.15	TTP	complex9.pdb.gz	113,114,115
10	0.01	2y3aA	0.344	7.84	0.040	0.565	0.12	GD9	complex10.pdb.gz	108,109,125,142

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Qiqige Wuyun, Yang Li, Quancheng Liu, Xiaogen Zhou, Yiheng Zhu, P. Lydia Freddolino, Yang Zhang. Integrating deep learning potentials with I-TASSER for single- and multi-domain protein structure prediction. Submitted. 2023.