D-I-TASSER P49356

D-I-TASSER results for P49356

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: P49356
Protein Name: Protein farnesyltransferase subunit beta
Gene Name: FNTB

Input Sequence in FASTA format

>P49356 (437 residues)
MASPSSFTYYCPPSSSPVWSEPLYSLRPEHARERLQDDSVETVTSIEQAKVEEKIQEVFS
SYKFNHLVPRLVLQREKHFHYLKRGLRQLTDAYECLDASRPWLCYWILHSLELLDEPIPQ
IVATDVCQFLELCQSPEGGFGGGPGQYPHLAPTYAAVNALCIIGTEEAYDIINREKLLQY
LYSLKQPDGSFLMHVGGEVDVRSAYCAASVASLTNIITPDLFEGTAEWIARCQNWEGGIG
GVPGMEAHGGYTFCGLAALVILKRERSLNLKSLLQWVTSRQMRFEGGFQGRCNKLVDGCY
SFWQAGLLPLLHRALHAQGDPALSMSHWMFHQQALQEYILMCCQCPAGGLLDKPGKSRDF
YHTCYCLSGLSIAQHFGSGAMLHDVVLGVPENALQPTHPVYNIGPDKVIQATTYFLQKPV
PGFEELKDETSAEPATD

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of DeepPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	P49356-D1	1-72,267-393	199		MASPSSFTYYCPPSSSPVWSEPLYSLRPEHARERLQDDSVETVTSIEQAKVEEKIQEVFSSYKFNHLVPRLVSLNLKSLLQWVTSRQMRFEGGFQGRCNKLVDGCYSFWQAGLLPLLHRALHAQGDPALSMSHWMFHQQALQEYILMCCQCPAGGLLDKPGKSRDFYHTCYCLSGLSIAQHFGSGAMLHDVVLGVPENA
2	P49356-D2	73-266,394-437	238		LQREKHFHYLKRGLRQLTDAYECLDASRPWLCYWILHSLELLDEPIPQIVATDVCQFLELCQSPEGGFGGGPGQYPHLAPTYAAVNALCIIGTEEAYDIINREKLLQYLYSLKQPDGSFLMHVGGEVDVRSAYCAASVASLTNIITPDLFEGTAEWIARCQNWEGGIGGVPGMEAHGGYTFCGLAALVILKRERLQPTHPVYNIGPDKVIQATTYFLQKPVPGFEELKDETSAEPATD

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.952

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of DeepPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	1ft1B	0.91	0.99	0.952	0.922	model1_1ft1B.pdb.gz
2	8t70B	0.86	2.25	0.382	0.902	model1_8t70B.pdb.gz
3	4l9pB	0.86	2.46	0.382	0.911	model1_4l9pB.pdb.gz
4	4ydeB	0.81	2.41	0.352	0.865	model1_4ydeB.pdb.gz
5	1dceB	0.69	2.10	0.284	0.735	model1_1dceB.pdb.gz
6	3draB	0.69	2.03	0.272	0.732	model1_3draB.pdb.gz
7	3sqcA	0.56	3.88	0.143	0.666	model1_3sqcA.pdb.gz
8	1tnzL1	0.37	1.93	0.285	0.391	model1_1tnzL1.pdb.gz
9	2qfcA	0.34	5.09	0.061	0.465	model1_2qfcA.pdb.gz
10	1zvrA2	0.29	6.69	0.036	0.458	model1_1zvrA2.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0008318	1.00	protein prenyltransferase activity
GO:1901363	0.59	heterocyclic compound binding
GO:0097159	0.59	organic cyclic compound binding
GO:0004660	0.56	protein farnesyltransferase activity
GO:0032555	0.54	purine ribonucleotide binding
GO:0032550	0.54	purine ribonucleoside binding
GO:0035639	0.50	purine ribonucleoside triphosphate binding
GO:0032561	0.50	guanyl ribonucleotide binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0018342	0.99	protein prenylation
GO:0065007	0.58	biological regulation
GO:0050794	0.57	regulation of cellular process
GO:0018343	0.56	protein farnesylation

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:1990234	1.00	transferase complex
GO:0044424	1.00	intracellular part
GO:0044444	0.88	cytoplasmic part
GO:0005965	0.57	protein farnesyltransferase complex

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.540	1dceB	0.693	2.10	0.284	0.735	2.5.1.60 2.5.1.-	300
2	0.431	1tnzL	0.686	1.95	0.283	0.721	2.5.1.59	99,151,154,198,200,205,248,250,254,297,300,303,353,359
3	0.166	1n4pB	0.685	1.97	0.283	0.721	2.5.1.59 2.5.1.-	300
4	0.067	1hzfA	0.505	3.68	0.117	0.604	3.4.21.43	NA
5	0.066	1fmiA	0.578	4.85	0.092	0.753	3.2.1.113	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.92	3eu5B	0.912	0.71	0.980	0.918	1.89	GBO	complex1.pdb.gz	99,102,106,202,248,250,251,291,294,300,303,361
2	0.88	1nl4B	0.909	0.92	0.983	0.918	1.92	HFP	complex2.pdb.gz	102,205,206,248,250,251,254,300,303,361
3	0.87	1o1tB	0.909	0.92	0.983	0.918	1.69	2NH	complex3.pdb.gz	98,99,102,106,151,152,202,205,206,248,250,254,297,299,300,303,361,362
4	0.85	1tn7B	0.917	1.30	0.975	0.931	1.01	III	complex4.pdb.gz	99,102,106,202,297,299,361,362
5	0.84	2h6iB	0.937	0.25	0.995	0.938	1.74	GER	complex5.pdb.gz	102,105,151,154,155,202,248,254,297,299,300,303
6	0.79	3e34B	0.917	1.29	0.975	0.931	1.49	ED1	complex6.pdb.gz	93,96,102,106,151,202,297,299,303,359,360,361,362
7	0.75	3dpyB	0.917	1.30	0.973	0.931	1.47	III	complex7.pdb.gz	98,99,102,149,151,152,202
8	0.73	1fppB	0.907	0.89	0.983	0.915	1.78	FPP	complex8.pdb.gz	102,106,202,250,254,303,361
9	0.67	1mzcB	0.930	0.23	1.000	0.931	0.88	FPP	complex9.pdb.gz	102,149,151,155,194,197,203,206,250
10	0.61	1tn6B	0.937	0.22	1.000	0.938	2.00	III	complex10.pdb.gz	81,92,93,95,98,99,102,106,149,151,152,202,297,299,357,358,359,361,362

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Qiqige Wuyun, Yang Li, Quancheng Liu, Xiaogen Zhou, Yiheng Zhu, P. Lydia Freddolino, Yang Zhang. Integrating deep learning potentials with I-TASSER for single- and multi-domain protein structure prediction. Submitted. 2023.