D-I-TASSER Q6P2C0-D1

D-I-TASSER results for Q6P2C0-D1

[Click result.zip to download all results on this page]

Input Sequence in FASTA format

>Q6P2C0 (416 residues)
MSFPRGSQTQKIKHPIGTRKGPLEVPPPTEKDWPKDDEQDHVLVDPDEELDSLPQPYRMI
NKLVNLLFDQSWEIIEERNALREAESSQIQPTVYPPLGEIQLNKMPNCMAVSQDYVFIGG
AKGFSIYNLYSAKQIYAWEKLKVDVTSIWATDLGNEILIAPVDEMGIIRLFYFYKEGLYL
VKAINEVDDTSKQTTCIKMEISQGGDFAAFLLQGAGDIWLDVYKLPKETWLKKLEHPQLT
SNPKKKVRQPQLNSLGPISADPLEMDANVSFKGDIKLSLPVYIMKIKPPKPVTGTTFKSP
LEVFAKIKDCYGLGSGQNHFIKDSQWEQQAEIFNASYKKYLDREWEEEPLSTATFYFLLP
SCLFAMPPEVKGPSGMACVLGIHWTRSHNFFLYSLNRTLKDKADPEGVWPCAAPIA

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MSFPRGSQTQKIKHPIGTRKGPLEVPPPTEKDWPKDDEQDHVLVDPDEELDSLPQPYRMINKLVNLLFDQSWEIIEERNALREAESSQIQPTVYPPLGEIQLNKMPNCMAVSQDYVFIGGAKGFSIYNLYSAKQIYAWEKLKVDVTSIWATDLGNEILIAPVDEMGIIRLFYFYKEGLYLVKAINEVDDTSKQTTCIKMEISQGGDFAAFLLQGAGDIWLDVYKLPKETWLKKLEHPQLTSNPKKKVRQPQLNSLGPISADPLEMDANVSFKGDIKLSLPVYIMKIKPPKPVTGTTFKSPLEVFAKIKDCYGLGSGQNHFIKDSQWEQQAEIFNASYKKYLDREWEEEPLSTATFYFLLPSCLFAMPPEVKGPSGMACVLGIHWTRSHNFFLYSLNRTLKDKADPEGVWPCAAPIA
Prediction	CCCCCCCHHHCCCCCSSSCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCSSSSCCCCCCCSSSCCCSSSSSCCCCSSSSSCCCCCSSSSSSCCCCSSSSSSSSSCCCSSSSSSSCCCCSSSSSSSSCCCSSSSSSSCCCCCCCCCCSSSSSSSCCCCCSSSSSSSCCCCSSSSSSSCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCSSSSSSCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCSSSSSCCCCSSCCCCCCCCCCCCCSSSSSSSCCCCCSSSSSCCCCCCCCCCCCCCCCCCCCCC
Confidence	99876541112446613325777899975124765455455225914515789973799999999999999999999999999885157787448843674279865035437669981589269997378874555305892699998642386589998247651567776568168998622467666652478899807995899999649807999996772578887640213455212444212468898888701034453225775558745899835998766666447789987653135436772024469999999999999888764223312566786389952771211576678899962699999759871687531444333458763156887679
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MSFPRGSQTQKIKHPIGTRKGPLEVPPPTEKDWPKDDEQDHVLVDPDEELDSLPQPYRMINKLVNLLFDQSWEIIEERNALREAESSQIQPTVYPPLGEIQLNKMPNCMAVSQDYVFIGGAKGFSIYNLYSAKQIYAWEKLKVDVTSIWATDLGNEILIAPVDEMGIIRLFYFYKEGLYLVKAINEVDDTSKQTTCIKMEISQGGDFAAFLLQGAGDIWLDVYKLPKETWLKKLEHPQLTSNPKKKVRQPQLNSLGPISADPLEMDANVSFKGDIKLSLPVYIMKIKPPKPVTGTTFKSPLEVFAKIKDCYGLGSGQNHFIKDSQWEQQAEIFNASYKKYLDREWEEEPLSTATFYFLLPSCLFAMPPEVKGPSGMACVLGIHWTRSHNFFLYSLNRTLKDKADPEGVWPCAAPIA
Prediction	75354454354351102135330613414355136453543203205532471330130013003300430052045355345455354513414232415237402000102000000013000001013252012035470400302013022200000011000000000143000001003444544452311403025512000000215430001002004620253053244344555534545444465442641545243334361302101000303214412544243135115424422100310111023521433441044114511554257432620001001112024233525335420000000003110000010243243445042001133417
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Contact, Hydrogen and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

Hydrogen bond networks

D-I-TASSER simulation is guided by the consensus contact map (left figure), distance map (middle figure) and Hydrogen bond network (right figure) derived based on confidence scores of DeepPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Top 10 threading templates used by D-I-TASSER

Rank

PDB
hit

ID1

ID2

Cov

Norm.
Zscore

Download
alignment

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

SS
Seq

CCCCCCCHHHCCCCCSSSCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCSSSSCCCCCCCSSSCCCSSSSSCCCCSSSSSCCCCCSSSSSSCCCCSSSSSSSSSCCCSSSSSSSCCCCSSSSSSSSCCCSSSSSSSCCCCCCCCCCSSSSSSSCCCCCSSSSSSSCCCCSSSSSSSCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCSSSSSSCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCSSSSSCCCCSSCCCCCCCCCCCCCSSSSSSSCCCCCSSSSSCCCCCCCCCCCCCCCCCCCCCC
MSFPRGSQTQKIKHPIGTRKGPLEVPPPTEKDWPKDDEQDHVLVDPDEELDSLPQPYRMINKLVNLLFDQSWEIIEERNALREAESSQIQPTVYPPLGEIQLNKMPNCMAVSQDYVFIGGAKGFSIYNLYSAKQIYAWEKLKVDVTSIWATDLGNEILIAPVDEMGIIRLFYFYKEGLYLVKAINEVDDTSKQTTCIKMEISQGGDFAAFLLQGAGDIWLDVYKLPKETWLKKLEHPQLTSNPKKKVRQPQLNSLGPISADPLEMDANVSFKGDIKLSLPVYIMKIKPPKPVTGTTFKSPLEVFAKIKDCYGLGSGQNHFIKDSQWEQQAEIFNASYKKYLDREWEEEPLSTATFYFLLPSCLFAMPPEVKGPSGMACVLGIHWTRSHNFFLYSLNRTLKDKADPEGVWPCAAPIA

5nuvA

0.07

0.05

1.99

0.36

CEthreader

---------------------------------------------------------------------------------------EVRVPATALCVFDAHDGEVNAVQFSSRLLATGGMDRVKLWEVAACEFKGSLSGSNAGITSIEFDS--AGSYLLAASNDFASRIWTV--DDYRLRHTLTGHSGK-----VLSAKFLLDNARIVSGSHDRTLKLWDLRSKVCIKTVFAGSSCNDIVCTEQCV---------------------MSGHFDKKIRFWDIRSESIVREMELLGKITALDLNPERTELLSCSRDDLLKVIDLRTNAIKQTFSAPGFKCGSDWTRVVFSPDGSYVAAGSAEGSLYIVEKVLSKQHSSSINAVAWSPSGSHVVSVDKGCKAVLWAQP----------

1gjqA

0.07

0.05

2.17

0.62

EigenThreader

MKAAEQYQGAASAVDPAHVVRTNGAPDMSCHGVLRKGATGK---------PLTPDITQQRGQQYLEALITYGITLMAKYIPEMRESWKQLNDLDLPNKVIDTGYAVHISRMSASGLLVIGDARIDMIDLWAKEPVAEIKI--GIEARSVESSKFKGYTIAGAYWPPQFAIMDGETLEPKQIVSTRGVDTQTYEPRVAAIIASHEHPEFIVNVK-ETGKVLLVNY---------------------------------------------KDID-----NLTVTSIGAAPFDPK---------------NHPQYAWKKVAELQ--------------------GGGSLFHPKSSHLYVDTTF---------NPDARISQSVAVFDLDAKYQVLPIAEWADLGEGAKKRGDGKNDSSA

6rlbD

0.11

0.09

3.41

0.56

FFAS-3D

----RWETKSCQTASIATASASAQARNHVDAQ-VQTEAPVPVSVQPPSQYD---------IPRLAAFLRRVEAMVIRELNKNWQSHATEQQQMVSCLYTLGYPPAVTSISWNGSVVACAYGRLDHGDWSTLKSFVCAWNEVPSAVLCLAFHPTQPSHVAGGLYS-GEVLVWDLSRLEDPLLWRTGLTDDTHTDPVSVVWLPEPGHSHRFQVLSVATDGKVLLWQG------IGVGQLQLTKLKKHPRGETEVGATASLAPAQFTFSPHGGPIYSVSCSPHVHLYSMLQAPPLTQLSLKYLFAVRWSPVRPLVFAAASGK-GDVQLFDLQKS----SQKPTVLIKQTQDESPVYCLEF-------------NSQQTQLLAAG---DAQGTVKVWQLSTEFTEQGPRE----------

3iiyA

0.10

0.07

2.79

0.77

SPARKS-K

--------------------------------------KCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADNFYTCAWTYDLLAVAGSRGIRIINPITMQCIKHYVGHGNAINELKFHP-RDPNLLLSVSKDHALRLWNIQTD--TLVAIFGGVEGHRDE--VLSADYDLLGEKIMSCGMDHS---LKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYRWLGDLISCENAIVCWKPGKMEDYSQCDIWYM--RFSTDFWQKMLALGN--------------------------------QVGKLYVWDLEVEDPHKAKCTTHHKCGAAIRQTSFSRDSSILIAVCD-------DASIRWDRL----

2ovpB

0.11

0.06

2.01

1.18

CNFpred

------------------------------------------------------SPWKSAYIRQHRIDTNWRRG------------------ELKSPKVLKGHHVITCLQFCGNRIVSGSDNTLKVWSAVTGKCLRTLV---GHTGGVWSSQMRDNIIISGSTD-RTLKVWNAE--TGECIHTLYGH-----TSTVRCMHLHE--KRVVSGSR---DATLRVWDIET-------------------------------------------GQCLHVLM-AVRCVQYDG----------------------RRVVSGAYD--------------------------------FMVKVWDPETCLHTLQG-------TNRVYSLQFDG-IHVVSGSLD-------TSIRVWDVETGNC

6rw8A

0.04

0.03

1.58

0.83

DEthreader

TAFNTDVAELMDPTSATDIKATG----------LGITGVAALIKLPTFDDWQ--LEATTTAASAYRDYIDNQEASIQLYVNDWETYNTVLIRIGQTGMAFDVANLQVISGYDL-YLIGYSQERIYYWRNGEWKKIEIPIN-VWQENIRPVIYKS-RLYLLWLEQTDYILKLSHRSWSSPFNFNVTILINKKASIGMYCSSDYEKD-VIIVYFHEKLPAREGMTINPD--M-----------------------------------------------TLSILTENDAKSLSATDYLAE-I--LSYISP-NLTFNPFNQVKVDI-----PLVSEFIIKDIVIE---------------ISL-HKMP-----S-G--TQYMQI---------YRTRLTEPNTEPMDNTSWNANPDA-Q

1fwxA1

0.07

0.06

2.47

0.50

MapAlign

------------------DGSVAPGQLDDYYGFWSSGQSGEMRILGIPSMRELMRVPVFNRCSATGWGQTNESVRIHE----RTMSERTKKFLAANGKRIHDNGDLHHVHMSFRFLFMNDKTRVARVRCDVMKCDAILEIPAKGIHGLRPQKWPRSNYVFCNGEDNVFTAVDAD--KWEVAWQVLV------SGNLDNCDADYEGKWAFSTSSKAEMDHIVVFNI--AEIEKAIAAGDYQELNGVK-----------------------VVDGRKEASSLFTRYIPIANNPHGCNMAPDKKHLCVA-----GKLSPTVTVLDVTRFDAVFYDAIRAYAGEKVDPIKDKLDVHYQPGHLKTVMGE-----TLDATNDWLVCLSKFSKLKPENDQLIDISGDKMVLVHDGPTFAEPHD

5c2vB

0.14

0.11

3.78

0.54

MUSTER

-GYIQGTHVKTLPGPFHIT-------------MSPDGS---FISNQS-------------GHSV-TFVD---------------------ARTQKVTGEVAVRVQPEASAVTPDFLYVCNADSVSVVDIQRKQEIKEIKVGDW-PSGIKISPDGK-TAYVACSGCMWNAIDVIDTGRMEKVRSIYTSDYG-----PRMVEISPDGKTLVAILDTVGSINRSVDF-----I--DIASGRVVENRVIHESSNLRDTPDGKYQTPKNWLPVCEAENGQVFTNNVTIIETKAGGKVARLPLDDLNNYDGNPPKGKYLGVRGMHRVTILDMDKVLGLVRSSTQEELDYLRDDLGLVRDYLVARVPTGLGPSSVCL-SPDGK--FCYAANYFSNNVTVIRTAVD------------------

1pevA1

0.10

0.03

1.17

0.69

HHsearch

--------------------------------------------------------------------------------------SEFSQTALFPSLPRTARGTAVVLGNTPAKIQYCNGTSVYTVPVGSLTDTEIYTEHSHQTTVAKTSP--SGYYCASGDVHGNVRIWDTTQTTHILKTTIPVF-----SGPVKDISWDSESKRIAAVGEGRERFHVFLFDTGTSN-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

6iamA

0.08

0.06

2.34

0.36

CEthreader

--------------------------------------------------------------------------------------TPVKPNYALKFTLAGHTKAVSSVKFSGEWLASSSAKLIKIWGAYDGKFEKTISGHKLGISDVAWS--SDSNLLVSASDDKTLKIWDVSSG--KCLKTLKGHSNY-----VFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYD----------------GLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNLVYIWNLQQKLQGHTDVVISTACHPTENIIASAAL----ENDKTIKLWKSDC---

(a)	ID1 is the number of template residues identical to query divided by number of aligned residues.
(b)	ID2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment and is highlighted in bold.
(e)	Download alignment lists the threading program used to identify the template, and provide the 3D structure of aligned regions of threading templates (threading[1-10].pdb.gz).
(f)	Template residues identical to query sequence are highlighted in color.

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.5534

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of DeepPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	6rw8A	0.49	6.33	0.055	0.748	model1_6rw8A.pdb.gz
2	1vw1A	0.48	6.33	0.057	0.738	model1_1vw1A.pdb.gz
3	6rw9A	0.46	6.44	0.074	0.728	model1_6rw9A.pdb.gz
4	4ui9I	0.46	5.11	0.054	0.606	model1_4ui9I.pdb.gz
5	6qzvA	0.42	4.86	0.026	0.538	model1_6qzvA.pdb.gz
6	6eojA	0.41	5.46	0.067	0.562	model1_6eojA.pdb.gz
7	4i0oA	0.41	5.27	0.061	0.548	model1_4i0oA.pdb.gz
8	1hj5A2	0.41	5.24	0.081	0.546	model1_1hj5A2.pdb.gz
9	5n4bA	0.41	5.87	0.040	0.579	model1_5n4bA.pdb.gz
10	2ovqB	0.41	5.92	0.070	0.570	model1_2ovqB.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0019903	0.13	protein phosphatase binding
GO:0004842	0.13	ubiquitin-protein transferase activity

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:1902099	0.36	regulation of metaphase/anaphase transition of cell cycle
GO:1901990	0.36	regulation of mitotic cell cycle phase transition
GO:0043161	0.36	proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0007091	0.36	metaphase/anaphase transition of mitotic cell cycle

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044464	0.92	cell part
GO:0044424	0.83	intracellular part
GO:0044446	0.75	intracellular organelle part
GO:0032991	0.75	macromolecular complex
GO:0044444	0.58	cytoplasmic part
GO:0043229	0.58	intracellular organelle
GO:0044428	0.50	nuclear part
GO:0043234	0.50	protein complex
GO:0043231	0.50	intracellular membrane-bounded organelle

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	1z68A	0.378	5.88	0.013	0.534	3.4.21.-	NA
2	0.060	1r9mB	0.384	6.00	0.039	0.555	3.4.14.5	NA
3	0.060	2htrA	0.385	5.25	0.062	0.529	3.2.1.18	NA
4	0.060	1utwF	0.392	5.63	0.054	0.548	3.2.1.26	NA
5	0.060	2aepA	0.384	5.47	0.072	0.536	3.2.1.18	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

No Binding sites was predicted

Click to view	Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Qiqige Wuyun, Yang Li, Quancheng Liu, Xiaogen Zhou, Yiheng Zhu, P. Lydia Freddolino, Yang Zhang. Integrating deep learning potentials with I-TASSER for single- and multi-domain protein structure prediction. Submitted. 2023.