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# D-I-TASSER results for Q8IWU6-D2

#### [Click result.zip to download all results on this page]

Input Sequence in FASTA format

>Q8IWU6 (108 residues) LRKKEESSKNIQQSNHLPKYERVKELCQQARYQTACEQPGQKWQCIEDTSGKLRIHKCKG PSDLLTVRQSTRNLYARGFHDKDKECSCRESGYRASRSQRKSQRQFLR

Predicted Secondary Structure

Sequence

20 40 60 80 100
| | | | |
LRKKEESSKNIQQSNHLPKYERVKELCQQARYQTACEQPGQKWQCIEDTSGKLRIHKCKGPSDLLTVRQSTRNLYARGFHDKDKECSCRESGYRASRSQRKSQRQFLR
Prediction
CCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCHHHCCCCCSSSSSCCCCCSSSSSCCCCCCCCCCCCCCCCCCCCCCCCCCCCSSCCCCCCCCCHHHHHHHHHHCC
Confidence
964335663235525685889999999987652332188862477745898657875368753234555654444345688886403588655667455432565409
H:Helix; S:Strand; C:Coil
Predicted Solvent Accessibility

Sequence | 20 40 60 80 100 | | | | | LRKKEESSKNIQQSNHLPKYERVKELCQQARYQTACEQPGQKWQCIEDTSGKLRIHKCKGPSDLLTVRQSTRNLYARGFHDKDKECSCRESGYRASRSQRKSQRQFLR
Prediction | 755467765436456414535404520545615420654455251265353413245163545334444444423443356645514157752545456454554348
| Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Contact, Hydrogen and Distance Map Used in D-I-TASSER simulation

Contact Map | Distance Map | Hydrogen bond networks

D-I-TASSER simulation is guided by the consensus contact map (left figure), distance map (middle figure) and Hydrogen bond network (right figure) derived based on confidence scores of DeepPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Top 10 threading templates used by D-I-TASSER

Rank | PDB hit | ID1 | ID2 | Cov | Norm. Zscore | Downloadalignment |  | 20 40 60 80 100 | | | | |
| SS Seq | CCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCHHHCCCCCSSSSSCCCCCSSSSSCCCCCCCCCCCCCCCCCCCCCCCCCCCCSSCCCCCCCCCHHHHHHHHHHCC LRKKEESSKNIQQSNHLPKYERVKELCQQARYQTACEQPGQKWQCIEDTSGKLRIHKCKGPSDLLTVRQSTRNLYARGFHDKDKECSCRESGYRASRSQRKSQRQFLR
1 | 2k23A | 0.04 | 0.04 | 1.90 | 0.44 | CEthreader |  | VTSILVRGQGCRYWIRTFVPSSRPGQFTLGNQVADTDYDQFAMVFFQKTSEYFKVTLYGRTKGLSDELKERFVSFAKSLGKDNNIVFSVPTDQCIDN-----------
2 | 4qiwC2 | 0.08 | 0.07 | 2.96 | 0.55 | EigenThreader |  | LIEYNEIQAIIDETVREYQKALKALEVA---RRIEGLSGVGKTIIRKEG-DEYVIYTEGSNFKQVLMLVADMMTLDGVIRPIGRHGIVGEKASVLARAAFHLFAAAER
3 | 2x57A | 0.15 | 0.12 | 4.03 | 0.34 | FFAS-3D |  | -------ENLYFQSRFHLEIQEEETKCSQTEKHKACSGVWDNITCWRPNVGETVTVPCPYSKAGNISKNCTSDGWSETFPDFVDACGYSDP-----------------
4 | 5ygbA1 | 0.08 | 0.07 | 2.89 | 0.76 | SPARKS-K |  | ---------GPQSKKLASMVQEMTSYYSSAENRAKHVYVGQIVAAVFKFDEKWYRAEIVDIMPNQYNPKEQVIDLYFVDYGDSEYISP--ADICELRTDFLTLRFQA-
5 | 4x08A | 0.11 | 0.06 | 1.99 | 0.72 | CNFpred |  | -------------------FANVVNVCGNQS--IRCPHNRTLNNCHRSR-FRVPLLHCDLINPGAQ---------------NISNCRYAD------------------
6 | 4zy8C | 0.01 | 0.01 | 1.03 | 0.83 | DEthreader |  | -----------------IPLNELKDYIFGSDSDKIKVVANIVLFTRIFYLRIAISCCVTVLVLCWISSFDQCENTLLYLATQFL-------HDWCIAAVLQTFQKIIP
7 | 4wj3M | 0.03 | 0.03 | 1.70 | 0.68 | MapAlign |  | YLVPSRTYPGHFFALPQSPQLFKQLLMVAGFLVDVAVFSGPAVAALRVDGDIVFFGADVCDALGALRIKVGHDLKLLTREWAPMWVVDFPMFEENDDGSLSAL-----
8 | 1xu6A | 0.15 | 0.11 | 3.72 | 0.60 | MUSTER |  | SHMLEVLTQKHKPAESQQQAAETEGSCNKKD-QNEC-KSPCKWH---DAENK----KCT----LDKEEAKKVADETAKDGKTGNTNTTGSS-----------------
9 | 5foeA | 0.17 | 0.15 | 4.86 | 0.89 | HHsearch |  | AHAAFIGSYIQREIIGFPISTTFNRLCPDT--EPTCEQP-AKWKIVYSSDSPWSEWTSCSTSCGNGIQQRGRSC-----DSLNNRCEGSSVQTRTCHI------QEC-
10 | 6yj1A | 0.05 | 0.05 | 2.25 | 0.39 | CEthreader |  | NYDEFCGGYHRAFDVYSNETNDVPAVTSGTVIEANDYGNFGGTFVIRDANDNDWIYGHLQRGSMRFQGDIIGLQGNSNYYDNPMSVHLHLQLRPKDAKKDEKSQVCSG
(a) | ID1 is the number of template residues identical to query divided by number of aligned residues.
(b) | ID2 is the number of template residues identical to query divided by query sequence length.
(c) | Cov is equal the number of aligned template residues divided by query sequence length.
(d) | Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment and is highlighted in bold.
(e) | Download alignment lists the threading program used to identify the template, and provide the 3D structure of aligned regions of threading templates (threading[1-10].pdb.gz).
(f) | Template residues identical to query sequence are highlighted in color.

Top 1 final models from D-I-TASSER

Click to view | Ranka | Download | Estimated TM-scoreb
| 1 | model1.pdb.gz | 0.4934
(a) | D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.
(b) | The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of DeepPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

| Top 10 structural analogs in PDB (as identified by TM-align)
Click to view | Rank | PDB Hit | TM-score | RMSDa | IDENa | Cov. | Download Alignment
--- | --- | --- | --- | --- | --- | --- | ---
| 1 | 3c7nA | 0.48 | 4.43 | 0.069 | 0.852 | model1_3c7nA.pdb.gz
| 2 | 4ixjA | 0.48 | 3.81 | 0.058 | 0.759 | model1_4ixjA.pdb.gz
| 3 | 4wxxA3 | 0.47 | 4.31 | 0.038 | 0.833 | model1_4wxxA3.pdb.gz
| 4 | 3zyyX | 0.47 | 4.14 | 0.032 | 0.824 | model1_3zyyX.pdb.gz
| 5 | 5lddB | 0.47 | 3.67 | 0.109 | 0.704 | model1_5lddB.pdb.gz
| 6 | 6kw5f | 0.47 | 3.99 | 0.019 | 0.787 | model1_6kw5f.pdb.gz
| 7 | 1nbwA | 0.47 | 3.86 | 0.048 | 0.768 | model1_1nbwA.pdb.gz
| 8 | 10mhA | 0.43 | 4.34 | 0.010 | 0.704 | model1_10mhA.pdb.gz
| 9 | 1krhA | 0.40 | 4.75 | 0.054 | 0.741 | model1_1krhA.pdb.gz
| 10 | 2fu3B1 | 0.39 | 4.69 | 0.031 | 0.694 | model1_2fu3B1.pdb.gz
(a) | Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b) | Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c) | RMSDa is the RMSD between residues that are structurally aligned by TM-align.
(d) | IDENa is the percentage sequence identity in the structurally aligned region.
(e) | Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)
GO term | CscoreGO | Name
GO:0008449 | 1.00 | N-acetylglucosamine-6-sulfatase activity
GO:0004065 | 1.00 | arylsulfatase activity
Download full result of the above consensus prediction.

| Click the graph to show a high resolution version.
(a) | CscoreGO is the confidence score of predicted GO terms. CscoreGO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.
(b) | The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by CscoreGO:
[0.4,0.5) | [0.5,0.6) | [0.6,0.7) | [0.7,0.8) | [0.8,0.9) | [0.9,1.0]

Biological Process (BP)

GO term | CscoreGO | Name
GO:0051239 | 1.00 | regulation of multicellular organismal process
GO:0048869 | 1.00 | cellular developmental process
GO:0048731 | 1.00 | system development
GO:0048523 | 1.00 | negative regulation of cellular process
GO:0044767 | 1.00 | single-organism developmental process
GO:0044707 | 1.00 | single-multicellular organism process
GO:0007166 | 1.00 | cell surface receptor signaling pathway
GO:0003008 | 1.00 | system process
GO:2000026 | 0.98 | regulation of multicellular organismal development
GO:0032879 | 0.98 | regulation of localization
GO:0045995 | 0.95 | regulation of embryonic development
GO:0030336 | 0.95 | negative regulation of cell migration
GO:0008152 | 0.95 | metabolic process
GO:0071704 | 0.94 | organic substance metabolic process
GO:1901135 | 0.93 | carbohydrate derivative metabolic process
GO:0044237 | 0.93 | cellular metabolic process
GO:0043170 | 0.93 | macromolecule metabolic process
GO:0048513 | 0.92 | animal organ development
GO:0030201 | 0.92 | heparan sulfate proteoglycan metabolic process
GO:0009968 | 0.92 | negative regulation of signal transduction
GO:0009967 | 0.92 | positive regulation of signal transduction
GO:0007169 | 0.92 | transmembrane receptor protein tyrosine kinase signaling pathway
GO:0060384 | 0.91 | innervation
GO:0060348 | 0.91 | bone development
GO:0051216 | 0.91 | cartilage development
GO:0048706 | 0.91 | embryonic skeletal system development
GO:0040037 | 0.91 | negative regulation of fibroblast growth factor receptor signaling pathway
GO:0035860 | 0.91 | glial cell-derived neurotrophic factor receptor signaling pathway
GO:0030177 | 0.91 | positive regulation of Wnt signaling pathway
GO:0014846 | 0.91 | esophagus smooth muscle contraction
GO:0002063 | 0.91 | chondrocyte development
GO:0001822 | 0.91 | kidney development
GO:2000027 | 0.89 | regulation of organ morphogenesis
GO:0050678 | 0.89 | regulation of epithelial cell proliferation
GO:0080090 | 0.88 | regulation of primary metabolic process
GO:0060255 | 0.88 | regulation of macromolecule metabolic process
GO:0031323 | 0.88 | regulation of cellular metabolic process
GO:0060686 | 0.87 | negative regulation of prostatic bud formation
GO:0048010 | 0.87 | vascular endothelial growth factor receptor signaling pathway
GO:0030513 | 0.87 | positive regulation of BMP signaling pathway
GO:0016525 | 0.87 | negative regulation of angiogenesis
GO:0001937 | 0.87 | negative regulation of endothelial cell proliferation
GO:0009653 | 0.82 | anatomical structure morphogenesis
GO:0048661 | 0.81 | positive regulation of smooth muscle cell proliferation
GO:0036022 | 0.81 | limb joint morphogenesis
GO:0007155 | 0.81 | cell adhesion
GO:0001502 | 0.81 | cartilage condensation

Download full result of the above consensus prediction.

| Click the graph to show a high resolution version.
(a) | CscoreGO is the confidence score of predicted GO terms. CscoreGO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.
(b) | The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by CscoreGO:
[0.4,0.5) | [0.5,0.6) | [0.6,0.7) | [0.7,0.8) | [0.8,0.9) | [0.9,1.0]

Cellular Component (CC)

GO term | CscoreGO | Name
GO:0016020 | 1.00 | membrane
GO:0009986 | 1.00 | cell surface
GO:0005783 | 1.00 | endoplasmic reticulum
GO:0005615 | 1.00 | extracellular space
GO:0045121 | 0.95 | membrane raft
GO:0005794 | 0.95 | Golgi apparatus

Download full result of the above consensus prediction.

| Click the graph to show a high resolution version.
(a) | CscoreGO is the confidence score of predicted GO terms. CscoreGO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.
(b) | The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by CscoreGO:
[0.4,0.5) | [0.5,0.6) | [0.6,0.7) | [0.7,0.8) | [0.8,0.9) | [0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

| Top 5 enzyme homologs in PDB
Click to view | Rank | CscoreEC | PDB Hit | TM-score | RMSDa | IDENa | Cov. | EC Number | Predicted Active Site Residues
--- | --- | --- | --- | --- | --- | --- | --- | --- | ---
| 1 | 0.060 | 1wd4A | 0.448 | 4.44 | 0.030 | 0.806 | 3.2.1.55 | NA
| 2 | 0.060 | 3drwA | 0.443 | 5.09 | 0.019 | 0.880 | 2.7.1.146 | NA
| 3 | 0.060 | 1odiA | 0.440 | 4.69 | 0.048 | 0.806 | 2.4.2.28 | NA
| 4 | 0.060 | 1nbwA | 0.465 | 3.86 | 0.048 | 0.768 | 4.2.1.30 | 100
| 5 | 0.060 | 1lrwC | 0.450 | 4.74 | 0.047 | 0.870 | 1.1.99.8 | NA
| Click on the radio buttons to visualize predicted active site residues.
(a) | CscoreEC is the confidence score for the Enzyme Commission (EC) number prediction. CscoreEC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b) | TM-score is a measure of global structural similarity between query and template protein.
(c) | RMSDa is the RMSD between residues that are structurally aligned by TM-align.
(d) | IDENa is the percentage sequence identity in the structurally aligned region.
(e) | Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

| Template proteins with similar binding site:
Click to view | Rank | CscoreLB | PDB Hit | TM-score | RMSDa | IDENa | Cov. | BS-score | Lig. Name | Download Complex | Predicted binding site residues
--- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | ---
No Binding sites was predicted
| Click on the radio buttons to visualize predicted binding site and residues.
(a) | CscoreLB is the confidence score of predicted binding site. CscoreLB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b) | BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c) | TM-score is a measure of global structural similarity between query and template protein.
(d) | RMSDa the RMSD between residues that are structurally aligned by TM-align.
(e) | IDENa is the percentage sequence identity in the structurally aligned region.
(f) | Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

#### [Click result.zip to download all results on this page]

References:
1. | Wei Zheng, Qiqige Wuyun, Yang Li, Quancheng Liu, Xiaogen Zhou, Yiheng Zhu, P. Lydia Freddolino, Yang Zhang. Integrating deep learning potentials with I-TASSER for single- and multi-domain protein structure prediction. Submitted. 2023.