D-I-TASSER Q9H477-D1

D-I-TASSER results for Q9H477-D1

[Click result.zip to download all results on this page]

Input Sequence in FASTA format

>Q9H477 (322 residues)
MAASGEPQRQWQEEVAAVVVVGSCMTDLVSLTSRLPKTGETIHGHKFFIGFGGKGANQCV
QAARLGAMTSMVCKVGKDSFGNDYIENLKQNDISTEFTYQTKDAATGTASIIVNNEGQNI
IVIVAGANLLLNTEDLRAAANVISRAKVMVCQLEITPATSLEALTMARRSGVKTLFNPAP
AIADLDPQFYTLSDVFCCNESEAEILTGLTVGSAADAGEAALVLLKRGCQVVIITLGAEG
CVVLSQTEPEPKHIPTEKVKAVDTTGAGDSFVGALAFYLAYYPNLSLEDMLNRSNFIAAV
SVQAAGTQSSYPYKKDLPLTLF

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MAASGEPQRQWQEEVAAVVVVGSCMTDLVSLTSRLPKTGETIHGHKFFIGFGGKGANQCVQAARLGAMTSMVCKVGKDSFGNDYIENLKQNDISTEFTYQTKDAATGTASIIVNNEGQNIIVIVAGANLLLNTEDLRAAANVISRAKVMVCQLEITPATSLEALTMARRSGVKTLFNPAPAIADLDPQFYTLSDVFCCNESEAEILTGLTVGSAADAGEAALVLLKRGCQVVIITLGAEGCVVLSQTEPEPKHIPTEKVKAVDTTGAGDSFVGALAFYLAYYPNLSLEDMLNRSNFIAAVSVQAAGTQSSYPYKKDLPLTLF
Prediction	CCCCCCCCCCCCCCCCCSSSSCCCSSSSSSSCCCCCCCCCSSSSCSSSSCCCCHHHHHHHHHHHCCCCSSSSSSSCCCHHHHHHHHHHHHHCCCCCCSSSCCCCCCCSSSSSSCCCCCSSSSSSCCCCCCCCHHHHHHHHHHHCCCCSSSSSCCCCHHHHHHHHHHHHHHCCSSSSSCCCCCCCCCHHHHHHCCSSSSCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCSSSSSSCCCCSSSSSCCCCSSSSSCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
Confidence	9987778777656788299987652768873278899994687071477138889999999998099669996745986899999999991991676789499886289999889999599993684559999999999987410989999089999999999999998199799818656665578899609989947999999979999984439999999998099989997287747999689987999678677755678626899999999999618999999999999999998476873025999999998629
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MAASGEPQRQWQEEVAAVVVVGSCMTDLVSLTSRLPKTGETIHGHKFFIGFGGKGANQCVQAARLGAMTSMVCKVGKDSFGNDYIENLKQNDISTEFTYQTKDAATGTASIIVNNEGQNIIVIVAGANLLLNTEDLRAAANVISRAKVMVCQLEITPATSLEALTMARRSGVKTLFNPAPAIADLDPQFYTLSDVFCCNESEAEILTGLTVGSAADAGEAALVLLKRGCQVVIITLGAEGCVVLSQTEPEPKHIPTEKVKAVDTTGAGDSFVGALAFYLAYYPNLSLEDMLNRSNFIAAVSVQAAGTQSSYPYKKDLPLTLF
Prediction	7545555455456523110000001020002044014341303054024111410000000001130301000012426104302620673433041033167230000000317713010000000012033620550262055020000001002500220041037370200000011246014300520200000040022004243644730440042017330623343211300000045653212031250521200000000000000000326723033004000000000013420140023263364157
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Contact, Hydrogen and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

Hydrogen bond networks

D-I-TASSER simulation is guided by the consensus contact map (left figure), distance map (middle figure) and Hydrogen bond network (right figure) derived based on confidence scores of DeepPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Top 10 threading templates used by D-I-TASSER

Rank

PDB
hit

ID1

ID2

Cov

Norm.
Zscore

Download
alignment

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

SS
Seq

CCCCCCCCCCCCCCCCCSSSSCCCSSSSSSSCCCCCCCCCSSSSCSSSSCCCCHHHHHHHHHHHCCCCSSSSSSSCCCHHHHHHHHHHHHHCCCCCCSSSCCCCCCCSSSSSSCCCCCSSSSSSCCCCCCCCHHHHHHHHHHHCCCCSSSSSCCCCHHHHHHHHHHHHHHCCSSSSSCCCCCCCCCHHHHHHCCSSSSCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCSSSSSSCCCCSSSSSCCCCSSSSSCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
MAASGEPQRQWQEEVAAVVVVGSCMTDLVSLTSRLPKTGETIHGHKFFIGFGGKGANQCVQAARLGAMTSMVCKVGKDSFGNDYIENLKQNDISTEFTYQTKDAATGTASIIVNNEGQNIIVIVAGANLLLNTEDLRAAANVISRAKVMVCQLEITPATSLEALTMARRSGVKTLFNPAPAIADLDPQFYTLSDVFCCNESEAEILTGLTVGSAADAGEAALVLLKRGCQVVIITLGAEGCVVLSQTEPEPKHIPTEKVKAVDTTGAGDSFVGALAFYLAYYPNLSLEDMLNRSNFIAAVSVQAAGTQSSYPYKKDLPLTLF

1rk2B

0.35

0.34

10.00

1.50

DEthreader

----------Q--NAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKGANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFVNGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNKTIVALNPAPARE-LPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIRTVLITLGSRGVWASVN--GEGQRVPGFRVQAVDTIAAGDTFNGALITALLEE--KPLPEAIRFAHAAAAIAVTRKGAQPSVPWREEIDAFLQ

2fv7A

1.00

0.96

26.78

2.17

SPARKS-K

--------------VAAVVVVGSCMTDLVSLTSRLPKTGETIHGHKFFIGFGGKGANQCVQAARLGAMTSMVCKVGKDSFGNDYIENLKQNDISTEFTYQTKDAATGTASIIVNNEGQNIIVIVAGANLLLNTEDLRAAANVISRAKVMVCQLEITPATSLEALTMARRSGVKTLFNPAPAIADLDPQFYTLSDVFCCNESEAEILTGLTVGSAADAGEAALVLLKRGCQVVIITLGAEGCVVLSQTEPEPKHIPTEKVKAVDTTGAGDSFVGALAFYLAYYPNLSLEDMLNRSNFIAAVSVQAAGTQSSYPYKKDLPLTLF

1rk2B

0.36

0.34

10.00

1.05

MapAlign

-------------NAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKGANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFVNGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNKTIVALNPAPARE-LPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIRTVLITLGSRGVWASVN--GEGQRVPGFRVQAVDTIAAGDTFNGALITALLE--EKPLPEAIRFAHAAAAIAVTRKGAQPSVPWREEIDAFL-

1rk2B

0.35

0.34

10.00

0.62

CEthreader

------------QNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKGANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFVNGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNKTIVALNPAPARE-LPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIRTVLITLGSRGVWASVN--GEGQRVPGFRVQAVDTIAAGDTFNGALITALLEE--KPLPEAIRFAHAAAAIAVTRKGAQPSVPWREEIDAFLD

2fv7A

1.00

0.96

26.78

1.91

MUSTER

--------------VAAVVVVGSCMTDLVSLTSRLPKTGETIHGHKFFIGFGGKGANQCVQAARLGAMTSMVCKVGKDSFGNDYIENLKQNDISTEFTYQTKDAATGTASIIVNNEGQNIIVIVAGANLLLNTEDLRAAANVISRAKVMVCQLEITPATSLEALTMARRSGVKTLFNPAPAIADLDPQFYTLSDVFCCNESEAEILTGLTVGSAADAGEAALVLLKRGCQVVIITLGAEGCVVLSQTEPEPKHIPTEKVKAVDTTGAGDSFVGALAFYLAYYPNLSLEDMLNRSNFIAAVSVQAAGTQSSYPYKKDLPLTLF

1rk2B

0.35

0.34

10.00

1.56

HHsearch

------------QNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKGANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFVNGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNKTIVALNPAPAR-ELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIRTVLITLGSRGVWASVNGE--GQRVPGFRVQAVDTIAAGDTFNGALITALLE--EKPLPEAIRFAHAAAAIAVTRKGAQPSVPWREEIDAFLD

2fv7A

1.00

0.96

26.78

3.07

FFAS-3D

--------------VAAVVVVGSCMTDLVSLTSRLPKTGETIHGHKFFIGFGGKGANQCVQAARLGAMTSMVCKVGKDSFGNDYIENLKQNDISTEFTYQTKDAATGTASIIVNNEGQNIIVIVAGANLLLNTEDLRAAANVISRAKVMVCQLEITPATSLEALTMARRSGVKTLFNPAPAIADLDPQFYTLSDVFCCNESEAEILTGLTVGSAADAGEAALVLLKRGCQVVIITLGAEGCVVLSQTEPEPKHIPTEKVKAVDTTGAGDSFVGALAFYLAYYPNLSLEDMLNRSNFIAAVSVQAAGTQSSYPYKKDLPLTLF

4x8fA

0.37

0.34

10.24

1.08

EigenThreader

--------------MNKLVVLGSVNADHVLQVPSFPRPGETLHGRNYQVIPGGKGANQAVAAARMQADVGFIACVGDDSFGINIRESFKLDGINTAGVKLQPNCPTGIAMIQVSDSGENSICISAEANAKLTAAAIEPDLAAIRDARYLLMQLETPLDGILKAAQEAKTAKTNVILNPAPARELPDE-LLKCVDLITPNETEAEVLTGITVYDDSSAQQAADALHCKGIEIVIITLGSKGVWLSQN----GRGIPGFVVKATDTTAAGDTFNGALVTGLLQ--EMPLESAIKFAHAAAAISVTRFGAQTSIPTRAEVEAFLA

2fv7A

1.00

0.96

26.78

2.55

CNFpred

--------------VAAVVVVGSCMTDLVSLTSRLPKTGETIHGHKFFIGFGGKGANQCVQAARLGAMTSMVCKVGKDSFGNDYIENLKQNDISTEFTYQTKDAATGTASIIVNNEGQNIIVIVAGANLLLNTEDLRAAANVISRAKVMVCQLEITPATSLEALTMARRSGVKTLFNPAPAIADLDPQFYTLSDVFCCNESEAEILTGLTVGSAADAGEAALVLLKRGCQVVIITLGAEGCVVLSQTEPEPKHIPTEKVKAVDTTGAGDSFVGALAFYLAYYPNLSLEDMLNRSNFIAAVSVQAAGTQSSYPYKKDLPLTLF

2fv7A

1.00

0.96

26.78

1.50

DEthreader

--------------VAAVVVVGSCMTDLVSLTSRLPKTGETIHGHKFFIGFGGKGANQCVQAARLGAMTSMVCKVGKDSFGNDYIENLKQNDISTEFTYQTKDAATGTASIIVNNEGQNIIVIVAGANLLLNTEDLRAAANVISRAKVMVCQLEITPATSLEALTMARRSGVKTLFNPAPAIADLDPQFYTLSDVFCCNESEAEILTGLTVGSAADAGEAALVLLKRGCQVVIITLGAEGCVVLSQTEPEPKHIPTEKVKAVDTTGAGDSFVGALAFYLAYYPNLSLEDMLNRSNFIAAVSVQAAGTQSSYPYKKDLPLTLF

(a)	ID1 is the number of template residues identical to query divided by number of aligned residues.
(b)	ID2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment and is highlighted in bold.
(e)	Download alignment lists the threading program used to identify the template, and provide the 3D structure of aligned regions of threading templates (threading[1-10].pdb.gz).
(f)	Template residues identical to query sequence are highlighted in color.

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.954

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of DeepPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	2fv7A	0.95	0.60	1.000	0.957	model1_2fv7A.pdb.gz
2	4x8fA	0.89	1.81	0.366	0.941	model1_4x8fA.pdb.gz
3	6ilrA	0.88	1.97	0.269	0.947	model1_6ilrA.pdb.gz
4	7vtdA	0.86	2.37	0.185	0.941	model1_7vtdA.pdb.gz
5	1rk2B	0.86	2.47	0.354	0.944	model1_1rk2B.pdb.gz
6	6whjA	0.86	2.33	0.292	0.947	model1_6whjA.pdb.gz
7	1vm7A	0.85	1.95	0.346	0.907	model1_1vm7A.pdb.gz
8	6znxA	0.85	2.55	0.380	0.922	model1_6znxA.pdb.gz
9	5zwyA	0.84	2.04	0.373	0.897	model1_5zwyA.pdb.gz
10	2a9zA	0.83	2.37	0.193	0.916	model1_2a9zA.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0003824	0.99	catalytic activity
GO:0016773	0.98	phosphotransferase activity, alcohol group as acceptor
GO:0016301	0.97	kinase activity
GO:0019200	0.95	carbohydrate kinase activity
GO:1901363	0.92	heterocyclic compound binding
GO:0097159	0.92	organic cyclic compound binding
GO:0035639	0.91	purine ribonucleoside triphosphate binding
GO:0032555	0.91	purine ribonucleotide binding
GO:0032550	0.91	purine ribonucleoside binding
GO:0005524	0.90	ATP binding
GO:0004747	0.90	ribokinase activity
GO:0043167	0.74	ion binding
GO:0046872	0.73	metal ion binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0008152	1.00	metabolic process
GO:0071704	0.99	organic substance metabolic process
GO:0044710	0.98	single-organism metabolic process
GO:0044238	0.98	primary metabolic process
GO:0044281	0.97	small molecule metabolic process
GO:0009987	0.97	cellular process
GO:0044237	0.96	cellular metabolic process
GO:0005975	0.96	carbohydrate metabolic process
GO:0044723	0.94	single-organism carbohydrate metabolic process
GO:0006796	0.93	phosphate-containing compound metabolic process
GO:0005996	0.92	monosaccharide metabolic process
GO:1901575	0.91	organic substance catabolic process
GO:0016310	0.91	phosphorylation
GO:0006014	0.91	D-ribose metabolic process
GO:0044712	0.90	single-organism catabolic process
GO:0044262	0.90	cellular carbohydrate metabolic process
GO:0044724	0.89	single-organism carbohydrate catabolic process
GO:0044282	0.89	small molecule catabolic process
GO:0046835	0.88	carbohydrate phosphorylation
GO:0046365	0.88	monosaccharide catabolic process
GO:0019303	0.87	D-ribose catabolic process
GO:0044763	0.51	single-organism cellular process

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044464	0.95	cell part
GO:0044424	0.93	intracellular part
GO:0044444	0.80	cytoplasmic part
GO:0043226	0.80	organelle
GO:0043227	0.78	membrane-bounded organelle
GO:0005829	0.59	cytosol
GO:0043229	0.57	intracellular organelle
GO:0043231	0.55	intracellular membrane-bounded organelle

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.419	3go6A	0.772	2.98	0.279	0.870	2.7.1.15	54,199,202,236,240,264,266,268,271,306
2	0.337	3hj6A	0.730	2.64	0.237	0.814	2.7.1.4	57,235,237,268,300
3	0.313	1ub0A	0.636	2.76	0.176	0.724	2.7.4.7	266
4	0.182	2jg1A	0.816	2.95	0.156	0.938	2.7.1.144	NA
5	0.178	3b3lA	0.797	2.22	0.191	0.876	2.7.1.3	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.56	1gqtC	0.861	2.41	0.356	0.941	1.21	RIB	complex1.pdb.gz	25,27,52,53,54,57,109,154,265,269
2	0.28	2c49B	0.812	2.45	0.213	0.894	1.33	ADN	complex2.pdb.gz	23,25,27,52,53,54,123
3	0.26	3n1cD	0.787	3.08	0.186	0.916	0.92	F6P	complex3.pdb.gz	27,54,55,58,107,109,152,153,269
4	0.23	1gqtA	0.862	2.39	0.356	0.941	1.35	ACP	complex4.pdb.gz	54,199,235,237,240,257,259,261,266,267,268,269,295,298
5	0.08	1rka0	0.888	1.87	0.356	0.941	1.43	III	complex5.pdb.gz	28,30,35,36,37,38,39,40,41,42,43,44,47,108,110,112,118,119,120,121,122,123,124

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Qiqige Wuyun, Yang Li, Quancheng Liu, Xiaogen Zhou, Yiheng Zhu, P. Lydia Freddolino, Yang Zhang. Integrating deep learning potentials with I-TASSER for single- and multi-domain protein structure prediction. Submitted. 2023.