D-I-TASSER Q9Y4A0

D-I-TASSER results for Q9Y4A0

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: Q9Y4A0
Protein Name: Jerky protein homolog-like
Gene Name: JRKL

Input Sequence in FASTA format

>Q9Y4A0 (524 residues)
MSGKRKRVVLTIKDKLDIIKKLEDGGSSKQLAVIYGIGETTVRDIRKNKEKIITYASSSD
STSLLAKRKSMKPSMYEELDRAMLEWFNQQRAKGNPISGPICAKRAEFFFYALGMDGDFN
PSAGWLTRFKQRHSIREINIRNERLNGDETAVEDFCNNFRDFIERENLQPEQIYNADETG
LFWKCLPSRISVIKGKCTVPGHKSIEERVTIMCCANATGLHKLKLCVVGKAKKPRSFKST
DTLNLPVSYFSQKGAWMDLSIFRQWFDKIFVPQVREYLRSKGLQEKAVLLLDNSPTHPNE
NVLRSDDGQIFAKYLPPNVASLIQPSDQGVIATMKRNYRAGLLQNNLEEGNDLKSFWKKL
TLLDALYEIAMAWNLVKPVTISRAWKKILPMVEEKESLDFDVEDISVATVAAILQHTKGL
ENVTTENLEKWLEVDSTEPGYEVLTDSEIIRRAQGQADESSENEEEEIELIPEKHINHAA
ALQWTENLLDYLEQQGDMILPDRLVIRKLRATIRNKQKMTKSSQ

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of DeepPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	Q9Y4A0-D1	1-137	137		MSGKRKRVVLTIKDKLDIIKKLEDGGSSKQLAVIYGIGETTVRDIRKNKEKIITYASSSDSTSLLAKRKSMKPSMYEELDRAMLEWFNQQRAKGNPISGPICAKRAEFFFYALGMDGDFNPSAGWLTRFKQRHSIRE
2	Q9Y4A0-D2	138-524	387		INIRNERLNGDETAVEDFCNNFRDFIERENLQPEQIYNADETGLFWKCLPSRISVIKGKCTVPGHKSIEERVTIMCCANATGLHKLKLCVVGKAKKPRSFKSTDTLNLPVSYFSQKGAWMDLSIFRQWFDKIFVPQVREYLRSKGLQEKAVLLLDNSPTHPNENVLRSDDGQIFAKYLPPNVASLIQPSDQGVIATMKRNYRAGLLQNNLEEGNDLKSFWKKLTLLDALYEIAMAWNLVKPVTISRAWKKILPMVEEKESLDFDVEDISVATVAAILQHTKGLENVTTENLEKWLEVDSTEPGYEVLTDSEIIRRAQGQADESSENEEEEIELIPEKHINHAAALQWTENLLDYLEQQGDMILPDRLVIRKLRATIRNKQKMTKSSQ

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.65

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of DeepPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	6tg9A	0.37	7.05	0.034	0.576	model1_6tg9A.pdb.gz
2	6r9tA	0.36	7.91	0.039	0.622	model1_6r9tA.pdb.gz
3	4ff1A	0.36	7.67	0.036	0.601	model1_4ff1A.pdb.gz
4	3ecqB	0.36	7.63	0.050	0.597	model1_3ecqB.pdb.gz
5	4zhjA	0.36	7.83	0.043	0.613	model1_4zhjA.pdb.gz
6	5gm6B	0.36	8.06	0.025	0.622	model1_5gm6B.pdb.gz
7	6cz7A	0.36	7.13	0.059	0.561	model1_6cz7A.pdb.gz
8	2amvA	0.36	6.79	0.040	0.538	model1_2amvA.pdb.gz
9	4f91B	0.36	7.13	0.036	0.548	model1_4f91B.pdb.gz
10	2ivfA	0.35	7.22	0.041	0.557	model1_2ivfA.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:1901363	0.66	heterocyclic compound binding
GO:0097159	0.66	organic cyclic compound binding
GO:0003676	0.59	nucleic acid binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0009987	0.78	cellular process
GO:0065007	0.74	biological regulation
GO:0050789	0.73	regulation of biological process
GO:0050794	0.71	regulation of cellular process
GO:0080090	0.69	regulation of primary metabolic process
GO:0060255	0.69	regulation of macromolecule metabolic process
GO:0031323	0.68	regulation of cellular metabolic process
GO:0006355	0.63	regulation of transcription, DNA-templated
GO:0044699	0.62	single-organism process
GO:0048523	0.60	negative regulation of cellular process
GO:0044763	0.59	single-organism cellular process
GO:0031324	0.59	negative regulation of cellular metabolic process
GO:0010605	0.59	negative regulation of macromolecule metabolic process
GO:0010629	0.55	negative regulation of gene expression
GO:0045892	0.54	negative regulation of transcription, DNA-templated

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044464	1.00	cell part
GO:0044424	0.99	intracellular part
GO:0043226	0.94	organelle
GO:0043231	0.93	intracellular membrane-bounded organelle
GO:0005634	0.92	nucleus

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	1e1yA	0.342	6.68	0.050	0.509	2.4.1.1	NA
2	0.060	3b9jI	0.159	5.54	0.054	0.218	1.17.1.4 1.17.3.2	NA
3	0.060	1ti6A	0.346	7.12	0.037	0.538	1.97.1.2	42
4	0.060	2vdcA	0.334	7.62	0.037	0.548	1.4.1.13	NA
5	0.060	2djiA	0.338	7.06	0.065	0.519	1.2.3.3	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.04	2skdA	0.356	6.77	0.052	0.536	0.33	PO4	complex1.pdb.gz	28,36,37,38
2	0.03	9gpbD	0.351	6.65	0.062	0.519	0.15	PLP	complex2.pdb.gz	26,42,43,45
3	0.01	3bd7A	0.344	6.69	0.053	0.513	0.36	CKB	complex3.pdb.gz	38,42,43
4	0.01	3mtdA	0.347	6.54	0.067	0.511	0.17	25E	complex4.pdb.gz	27,29,36,39
5	0.01	1z62A	0.342	6.78	0.075	0.513	0.27	IAA	complex5.pdb.gz	19,36,38,39
6	0.01	1wutA	0.347	6.57	0.053	0.511	0.16	BN2	complex6.pdb.gz	36,37,44
7	0.01	1gpaD	0.349	7.02	0.037	0.544	0.12	PLP	complex7.pdb.gz	15,26,39
8	0.01	8gpbA	0.351	6.78	0.053	0.534	0.12	PLP	complex8.pdb.gz	26,39,43
9	0.01	3ms4A	0.344	6.65	0.058	0.511	0.12	21N	complex9.pdb.gz	32,33,34,38
10	0.01	1pygA	0.355	6.75	0.035	0.536	0.11	AMP	complex10.pdb.gz	37,38,42

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Qiqige Wuyun, Yang Li, Quancheng Liu, Xiaogen Zhou, Yiheng Zhu, P. Lydia Freddolino, Yang Zhang. Integrating deep learning potentials with I-TASSER for single- and multi-domain protein structure prediction. Submitted. 2023.