StarFunc result for job id P04818

User Input

>input (313 residues)
MPVAGSELPRRPLPPAAQERDAEPRPPHGELQYLGQIQHILRCGVRKDDRTGTGTLSVFG
MQARYSLRDEFPLLTTKRVFWKGVLEELLWFIKGSTNAKELSSKGVKIWDANGSRDFLDS
LGFSTREEGDLGPVYGFQWRHFGAEYRDMESDYSGQGVDQLQRVIDTIKTNPDDRRIIMC
AWNPRDLPLMALPPCHALCQFYVVNSELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHI
TGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVEKIDDFKAEDFQIEG
YNPHPTIKMEMAV

Proteins with similar structure

Top structural analogs (as identified by Foldseek and TM-align)

Rank	Structure template	TM-score1	TM-score2	Foldseek E-value	Length	ID1	ID2	Download	Description
1	AFDB:P04818	1.000	1.000	1.333E-72	313	1.000	1.000	P04818.pdb.gz	TYSY_HUMAN Thymidylate synthase OS=Homo sapiens OX=9606 GN=TYMS PE=1 SV=3
2	AFDB:P45352	0.918	0.936	3.625E-63	307	0.863	0.879	P45352.pdb.gz	TYSY_RAT Thymidylate synthase OS=Rattus norvegicus OX=10116 GN=Tyms PE=1 SV=1
3	PDB:4eb4B	0.914	0.951	9.377E-61	301	0.856	0.890	4eb4B.pdb.gz	Crystal structure of mouse thymidylate synthase in tertiary complex with dUMP and raltitrexed reveals N-terminus architecture and two different active site conformations.
4	AFDB:Q7ZUI7	0.910	0.893	3.400E-60	319	0.754	0.740	Q7ZUI7.pdb.gz
5	AFDB:Q9N588	0.908	0.910	6.762E-54	312	0.620	0.622	Q9N588.pdb.gz	Q9N588_CAEEL Thymidylate synthase OS=Caenorhabditis elegans OX=6239 GN=tyms-1 PE=1 SV=1
6	PDB:4kpwA	0.809	0.953	1.481E-53	265	0.843	0.996	4kpwA.pdb.gz
7	AFDB:Q05763	0.915	0.509	7.825E-53	565	0.607	0.336	Q05763.pdb.gz	DRTS2_ARATH Bifunctional dihydrofolate reductase-thymidylate synthase 2 OS=Arabidopsis thaliana OX=3702 GN=THY-2 PE=2 SV=2
8	AFDB:Q05762	0.909	0.549	4.004E-52	519	0.597	0.360	Q05762.pdb.gz	DRTS1_ARATH Bifunctional dihydrofolate reductase-thymidylate synthase 1 OS=Arabidopsis thaliana OX=3702 GN=THY-1 PE=1 SV=2
9	AFDB:P06785	0.902	0.929	2.687E-50	304	0.591	0.609	P06785.pdb.gz	TYSY_YEAST Thymidylate synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CDC21 PE=1 SV=1
10	AFDB:Q582G3	0.899	0.536	1.591E-48	527	0.546	0.324	Q582G3.pdb.gz	Q582G3_TRYB2 Bifunctional dihydrofolate reductase-thymidylate synthase OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.1) OX=185431 GN=Tb07.10C21.550 PE=3 SV=1
11	AFDB:Q9UTI7	0.908	0.457	1.694E-48	625	0.575	0.288	Q9UTI7.pdb.gz	TYSY_SCHPO Probable thymidylate synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC15E1.04 PE=3 SV=1
12	AFDB:Q8I1R6	0.905	0.468	1.432E-47	608	0.508	0.262	Q8I1R6.pdb.gz	Q8I1R6_PLAF7 Bifunctional dihydrofolate reductase-thymidylate synthase OS=Plasmodium falciparum (isolate 3D7) OX=36329 GN=PF3D7_0417200 PE=1 SV=1
13	PDB:7ctzA	0.893	0.526	3.069E-45	534	0.505	0.296	7ctzA.pdb.gz	Discovery of new non-pyrimidine scaffolds as Plasmodium falciparum DHFR inhibitors by fragment-based screening.
14	PDB:3egyX	0.715	0.950	3.705E-45	234	0.744	0.996	3egyX.pdb.gz
15	PDB:2a9wA	0.807	0.955	1.503E-40	264	0.460	0.545	2a9wA.pdb.gz	The structure of Cryptococcus neoformans thymidylate synthase suggests strategies for using target dynamics for species-specific inhibition.
16	PDB:4fogD	0.814	0.967	1.972E-39	263	0.450	0.536	4fogD.pdb.gz	Crystal structure of binary and ternary complexes of thymidylate synthase (ThyA) from Mycobacterium tuberculosis: Insights into the selectivity and mode of inhibition
17	PDB:1b02A	0.773	0.865	2.579E-32	279	0.342	0.384	1b02A.pdb.gz	Crystal structure of thymidylate synthase A from Bacillus subtilis.
18	PDB:1tisA	0.771	0.840	1.375E-29	286	0.406	0.444	1tisA.pdb.gz
19	PDB:6z6bPPP	0.156	0.032	1.033	2036	0.061	0.009	6z6bPPP.pdb.gz	Pre-initiation and elongation structures of full-length La Crosse virus polymerase reveal functionally important conformational changes.
20	PDB:3ax7A	0.103	0.034	1.171	1225	0.029	0.007	3ax7A.pdb.gz	Protein conformational gating of enzymatic activity in xanthine oxidoreductase

(a)	The query structure is shown in cartoon, while the structural analog is displayed using a backbone trace.
(b)	Ranking of proteins is based on E-value of Foldseek alignment.
(c)	TM-score1 and ID1 are TM-score and sequence identity normalized by query sequence length.
(d)	TM-score2 and ID2 are TM-score and sequence identity normalized by template sequence length.

Proteins with similar sequence

Top sequence homologs in UniProt

Rank	Template	E-value	Bit-score	Length	ID1	ID2	Description
1	P04818	0.0	652	313	1.000	1.000	TYSY_HUMAN Thymidylate synthase OS=Homo sapiens OX=9606 GN=TYMS PE=1 SV=3
2	P07607	0.0	580	307	0.888	0.906	TYSY_MOUSE Thymidylate synthase OS=Mus musculus OX=10090 GN=Tyms PE=1 SV=1
3	P45352	0.0	576	307	0.882	0.899	TYSY_RAT Thymidylate synthase OS=Rattus norvegicus OX=10116 GN=Tyms PE=1 SV=1
4	Q6P045	0.0	509	318	0.754	0.742	Q6P045_DANRE Thymidylate synthase OS=Danio rerio OX=7955 GN=zgc:56465 PE=1 SV=1
5	Q9N588	6.83e-147	417	312	0.620	0.622	Q9N588_CAEEL Thymidylate synthase OS=Caenorhabditis elegans OX=6239 GN=tyms-1 PE=1 SV=1
6	Q05763	1.20e-141	413	565	0.601	0.333	DRTS2_ARATH Bifunctional dihydrofolate reductase-thymidylate synthase 2 OS=Arabidopsis thaliana OX=3702 GN=THY-2 PE=2 SV=2
7	Q05762	7.82e-141	409	519	0.597	0.360	DRTS1_ARATH Bifunctional dihydrofolate reductase-thymidylate synthase 1 OS=Arabidopsis thaliana OX=3702 GN=THY-1 PE=1 SV=2
8	P06785	1.71e-130	375	304	0.588	0.605	TYSY_YEAST Thymidylate synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CDC21 PE=1 SV=1
9	Q582G3	6.03e-126	372	527	0.550	0.326	Q582G3_TRYB2 Bifunctional dihydrofolate reductase-thymidylate synthase OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.1) OX=185431 GN=Tb07.10C21.550 PE=3 SV=1
10	Q9UTI7	1.41e-122	366	625	0.581	0.291	TYSY_SCHPO Probable thymidylate synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC15E1.04 PE=3 SV=1
11	Q8I1R6	1.04e-119	358	608	0.514	0.265	Q8I1R6_PLAF7 Bifunctional dihydrofolate reductase-thymidylate synthase OS=Plasmodium falciparum (isolate 3D7) OX=36329 GN=PF3D7_0417200 PE=1 SV=1
12	P0A884	1.50e-95	285	264	0.460	0.545	TYSY_ECOLI Thymidylate synthase OS=Escherichia coli (strain K12) OX=83333 GN=thyA PE=1 SV=1
13	P9WFR9	6.93e-92	276	266	0.450	0.530	TYSY_MYCTU Thymidylate synthase ThyA OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=thyA PE=1 SV=2
14	P00471	4.10e-75	234	286	0.419	0.458	TYSY_BPT4 Thymidylate synthase OS=Enterobacteria phage T4 OX=10665 GN=TD PE=1 SV=1
15	Q6QGJ5	1.12e-71	224	279	0.409	0.459	THY_BPT5 Probable thymidylate synthase OS=Escherichia phage T5 OX=2695836 GN=thy PE=1 SV=1
16	P0CI79	2.86e-54	180	279	0.342	0.384	TYSY1_BACSU Thymidylate synthase 1 OS=Bacillus subtilis (strain 168) OX=224308 GN=thyA1 PE=1 SV=1
17	P67049	9.62e-19	86.3	279	0.262	0.294	TYSY_STRPN Thymidylate synthase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=thyA PE=1 SV=1
18	P31654	6.72e-06	49.7	383	0.093	0.076	DUHM_BPSP1 Deoxyuridylate hydroxymethyltransferase OS=Bacillus phage SP01 OX=2884427 PE=1 SV=1
19	Q1PE30	0.42	34.7	764	0.115	0.047	Q1PE30_ARATH Kinase with adenine nucleotide alpha hydrolases-like domain-containing protein OS=Arabidopsis thaliana OX=3702 GN=At4g31230 PE=1 SV=1
20	P25888	0.73	33.9	454	0.086	0.059	RHLE_ECOLI ATP-dependent RNA helicase RhlE OS=Escherichia coli (strain K12) OX=83333 GN=rhlE PE=1 SV=3

(a)	Ranking of proteins is based on the E-value of a BLASTp alignment.
(b)	ID1 and ID2 are sequence identities normalized by the query and template sequence lengths, respectively.

Pfam families

Top Pfam family matches

Rank	Pfam family	E-value	Score	Description
1	PF00303 Thymidylat_synt	2.8e-115	395.7	Thymidylate synthase

(a)	Ranking of Pfam families is based on the E-value provided by HMMsearch.

Protein-protein interaction (PPI) partners

Top PPI partners to 9606.ENSP00000315644

Rank	PPI partner	STRING score	Description
1	9606.ENSP00000396308	0.999	Dihydrofolate reductase
2	9606.ENSP00000370376	0.998	Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
3	9606.ENSP00000301634	0.995	Thymidine kinase, cytosolic
4	9606.ENSP00000349576	0.993	Deoxycytidylate deaminase
5	9606.ENSP00000365777	0.993	Methylenetetrahydrofolate reductase
6	9606.ENSP00000304802	0.992	Thymidylate kinase
7	9606.ENSP00000318868	0.991	Serine hydroxymethyltransferase, cytosolic
8	9606.ENSP00000377768	0.989	Dihydrofolate reductase 2, mitochondrial
9	9606.ENSP00000216605	0.988	C-1-tetrahydrofolate synthase, cytoplasmic
10	9606.ENSP00000265056	0.986	DNA replication licensing factor MCM2
11	9606.ENSP00000333667	0.985	Serine hydroxymethyltransferase, mitochondrial
12	9606.ENSP00000378699	0.985	Cyclin-dependent kinase 1
13	9606.ENSP00000300738	0.983	Ribonucleoside-diphosphate reductase large subunit
14	9606.ENSP00000362344	0.980	Folylpolyglutamate synthase, mitochondrial
15	9606.ENSP00000353770	0.973	Ribonucleoside-diphosphate reductase subunit M2
16	9606.ENSP00000377617	0.973	Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
17	9606.ENSP00000262105	0.971	DNA replication licensing factor MCM4
18	9606.ENSP00000368458	0.964	Proliferating cell nuclear antigen
19	9606.ENSP00000379108	0.961	Probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2
20	9606.ENSP00000251496	0.960	Condensin complex subunit 3

A higher STRING score indicates a greater likelihood of interaction.
The query and PPI partners are shown as grey oval and white rectangles, respectively.
Interactions between query and PPI partners are shown by solid black lines. Interactions among PPI partners are by dashed grey lines.
Values in the rectangles are the STRING scores between query and each corresponding PPI partner

Predicted Gene Ontology (GO) Terms

GO term	C-score	Name
GO:0004799	0.999	thymidylate synthase activity
GO:0005488	0.977	binding
GO:0097159	0.882	organic cyclic compound binding
GO:0005515	0.874	protein binding
GO:1901363	0.872	heterocyclic compound binding
GO:0003676	0.494	nucleic acid binding
GO:0043167	0.356	ion binding

C-score is the confidence score of a predicted GO term. Its value ranges from (0,1], where a higher value indicates a higher confidence prediction.
If a child GO term and its parent term have the same C-score, only the child term is shown in this table.

The graph shows the predicted terms for the Molecular Function (MF) GO aspect. GO terms shown in the table are colored by C-score in the graph:

[0.3,0.4)

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

GO term	C-score	Name
GO:0044238	0.998	primary metabolic process
GO:0006807	0.998	nitrogen compound metabolic process
GO:1901360	0.995	organic cyclic compound metabolic process
GO:0046483	0.995	heterocycle metabolic process
GO:0006725	0.995	cellular aromatic compound metabolic process
GO:1901564	0.986	organonitrogen compound metabolic process
GO:0006139	0.986	nucleobase-containing compound metabolic process
GO:0009129	0.975	pyrimidine nucleoside monophosphate metabolic process
GO:0006221	0.975	pyrimidine nucleotide biosynthetic process
GO:0009265	0.975	2'-deoxyribonucleotide biosynthetic process
GO:0009162	0.969	deoxyribonucleoside monophosphate metabolic process
GO:0009219	0.969	pyrimidine deoxyribonucleotide metabolic process
GO:0009124	0.969	nucleoside monophosphate biosynthetic process
GO:0006575	0.918	cellular modified amino acid metabolic process
GO:0042558	0.916	pteridine-containing compound metabolic process
GO:0046653	0.856	tetrahydrofolate metabolic process
GO:0065007	0.780	biological regulation
GO:0050789	0.743	regulation of biological process
GO:0050794	0.730	regulation of cellular process
GO:0019222	0.462	regulation of metabolic process
GO:0050896	0.399	response to stimulus
GO:0060255	0.391	regulation of macromolecule metabolic process
GO:0080090	0.372	regulation of primary metabolic process
GO:0051171	0.336	regulation of nitrogen compound metabolic process
GO:0031323	0.335	regulation of cellular metabolic process

C-score is the confidence score of a predicted GO term. Its value ranges from (0,1], where a higher value indicates a higher confidence prediction.
If a child GO term and its parent term have the same C-score, only the child term is shown in this table.

The graph shows the predicted terms for the Biological Process (BP) GO aspect. GO terms shown in the table are colored by C-score in the graph:

[0.3,0.4)

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

GO term	C-score	Name
GO:0005634	1.000	nucleus
GO:0005737	0.999	cytoplasm
GO:0070013	0.996	intracellular organelle lumen
GO:0031981	0.949	nuclear lumen
GO:0005654	0.937	nucleoplasm
GO:0016020	0.889	membrane
GO:0005739	0.888	mitochondrion
GO:0043232	0.816	intracellular non-membrane-bounded organelle
GO:0005829	0.787	cytosol

C-score is the confidence score of a predicted GO term. Its value ranges from (0,1], where a higher value indicates a higher confidence prediction.
If a child GO term and its parent term have the same C-score, only the child term is shown in this table.

The graph shows the predicted terms for the Cellular Component (CC) GO aspect. GO terms shown in the table are colored by C-score in the graph:

[0.3,0.4)

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted reactions (EC numbers and Rhea reactions)

Reaction	C-score	Name
RHEA:12104	0.999	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8-dihydrofolate + dTMP
EC:2.1.1.45	0.999	thymidylate synthase.

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