I would like to run the following sequence through D-I-TASSER. Unfortunately the length is 1083. Is there a way around the 750 limit in the text paste box? I tried uploading the sequence as a .fasta file from my computer with no luck, I was told the text paste box was empty.
>sp|Q9ULD8|KCNH3_HUMAN Potassium voltage-gated channel subfamily H member 3 OS=Homo sapiens OX=9606 GN=KCNH3 PE=1 SV=2
MPAMRGLLAPQNTFLDTIATRFDGTHSNFVLGNAQVAGLFPVVYCSDGFCDLTGFSRAEV
MQRGCACSFLYGPDTSELVRQQIRKALDEHKEFKAELILYRKSGLPFWCLLDVIPIKNEK
GEVALFLVSHKDISETKNRGGPDRWKETGGGRRRYGRARSKGFNANRRRSRAVLYHLSGH
LQKQPKGKHKLNKGVFGEKPNLPEYKVAAIRKSPFILLHCGALRATWDGFILLATLYVAV
TVPYSVCVSTAREPSAARGPPSVCDLAVEVLFILDIVLNFRTTFVSKSGQVVFAPKSICL
HYVTTWFLLDVIAALPFDLLHAFKVNVYFGAHLLKTVRLLRLLRLLPRLDRYSQYSAVVL
TLLMAVFALLAHWVACVWFYIGQREIESSESELPEIGWLQELARRLETPYYLVGRRPAGG
NSSGQSDNCSSSSEANGTGLELLGGPSLRSAYITSLYFALSSLTSVGFGNVSANTDTEKI
FSICTMLIGALMHAVVFGNVTAIIQRMYARRFLYHSRTRDLRDYIRIHRIPKPLKQRMLE
YFQATWAVNNGIDTTELLQSLPDELRADIAMHLHKEVLQLPLFEAASRGCLRALSLALRP
AFCTPGEYLIHQGDALQALYFVCSGSMEVLKGGTVLAILGKGDLIGCELPRREQVVKANA
DVKGLTYCVLQCLQLAGLHDSLALYPEFAPRFSRGLRGELSYNLGAGGGSAEVDTSSLSG
DNTLMSTLEEKETDGEQGPTVSPAPADEPSSPLLSPGCTSSSSAAKLLSPRRTAPRPRLG
GRGRPGRAGALKAEAGPSAPPRALEGLRLPPMPWNVPPDLSPRVVDGIEDGCGSDQPKFS
FRVGQSGPECSSSPSPGPESGLLTVPHGPSEARNTDTLDKLRQAVTELSEQVLQMREGLQ
SLRQAVQLVLAPHREGPCPRASGEGPCPASTSGLLQPLCVDTGASSYCLQPPAGSVLSGT
WPHPRPGPPPLMAPWPWGPPASQSSPWPRATAFWTSTSDSEPPASGDLCSEPSTPASPPP
SEEGARTGPAEPVSQAEATSTGEPPPGSGGLALPWDPHSLEMVLIGCHGSGTVQWTQEEG
TGV
Any help is greatly appreciated. Thank you.
Options for uploading a longer sequence to D-I-TASSER?
Moderator: robpearc
Re: Options for uploading a longer sequence to D-I-TASSER?
Dear user,
Due to the resource limitation, we limited the sequence to 750AA current.
Please find our pre-build model by D-I-TASSER for your target protein. https://zhanggroup.org/HPmod/data/Q9ULD8/result.zip.
for your case, I think the C-terminal may not be that confident, you can put the C-terminal sequence (cut from the whole sequence) to the D-I-TASSER server and select the AF2 distance and IMG option, to see if the C-terminal can be improved.
Best
IT Team
Due to the resource limitation, we limited the sequence to 750AA current.
Please find our pre-build model by D-I-TASSER for your target protein. https://zhanggroup.org/HPmod/data/Q9ULD8/result.zip.
for your case, I think the C-terminal may not be that confident, you can put the C-terminal sequence (cut from the whole sequence) to the D-I-TASSER server and select the AF2 distance and IMG option, to see if the C-terminal can be improved.
Best
IT Team